A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

Garry Mcdowell; Richard Body; Cliona Kirwan; Ged Byrne; Mark Slevin

doi:10.1007/978-94-017-9716-0_35

A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

Garry Mcdowell
, Richard Body
, Cliona Kirwan
, Ged Byrne
, Mark Slevin

Research output: Chapter in Book/Conference proceeding › Chapter › peer-review

Abstract

In this chapter the authors describe a protocol that can be applied to design and validate an ELISA technique using commercially available reagents. This often proves an economical alternative to purchasing off-the-shelf-kits. In addition, this protocol allows custom validation of the ELISA to a specific purpose. We have illustrated our protocol using VCAM as an example, however the principles and techniques described can equally be applied to all ELISA based techniques.

Original language	English
Title of host publication	Handbook of Vascular Biology Techniques
Editors	Mark Slevin, Garry McDowell
Place of Publication	Netherlands
Publisher	Springer Nature
Pages	453-463
ISBN (Electronic)	978-94-017-9716-0
ISBN (Print)	978-94-017-9715-3
DOIs	https://doi.org/10.1007/978-94-017-9716-0_35
Publication status	Published - 2015

Keywords

ELISA
Reagents
Development
Validation
VCAM
Angiogenesis markers
Serum

Research Highlights, Institutes and Platforms

Manchester Cancer Research Centre

Access to Document

10.1007/978-94-017-9716-0_35

https://books.google.co.uk/books?id=PLO1BwAAQBAJ&pg=PA453&lpg=PA453&dq=G.+McDowell,+R.+Body,+Kirwan.+C,+Byrne.+G,+Slevin.+M.+A+scheme+for+the+development+and+validation+of+enzyme+linked+immunosorbent+assays+(ELISA)+for+measurement+of+angiogenic+biomarkers+in+human+blood.+In+Handbook+of+Vascular+Biology+Techniques&source=bl&ots=6N_5ZBauGr&sig=DbtS-htcmgpN5jPv0X04Hiz6Ehw&hl=en&sa=X&ved=0ahUKEwj_mIf6nNbSAhUGQpAKHTxvC1IQ6AEIGjAA#v=onepage&q=G.%20McDowell%2C%20R.%20Body%2C%20Kirwan.%20C%2C%20Byrne.%20G%2C%20Slevin.%20M.%20A%20scheme%20for%20the%20development%20and%20validation%20of%20enzyme%20linked%20immunosorbent%20assays%20(ELISA)%20for%20measurement%20of%20angiogenic%20biomarkers%20in%20human%20blood.%20In%20Handbook%20of%20Vascular%20Biology%20Techniques&f=false

Cite this

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A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood. / Mcdowell, Garry; Body, Richard ; Kirwan, Cliona et al.
Handbook of Vascular Biology Techniques. ed. / Mark Slevin; Garry McDowell. Netherlands: Springer Nature, 2015. p. 453-463.

Research output: Chapter in Book/Conference proceeding › Chapter › peer-review

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AU - Slevin, Mark

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PB - Springer Nature

CY - Netherlands

ER -

A scheme for the development and validation of enzyme linked immunosorbent assays (ELISA) for measurement of angiogenic biomarkers in human blood.

Abstract

Keywords

Research Highlights, Institutes and Platforms

Access to Document

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Cite this