A simple histochemical method for the identification of cytotrophoblasts in tissue sections

Carolyn Jones; Chris Whittle; John Aplin

doi:10.1016/j.placenta.2016.03.014

A simple histochemical method for the identification of cytotrophoblasts in tissue sections

Carolyn Jones, Chris Whittle, John Aplin

Research output: Contribution to journal › Article › peer-review

Abstract

A simple method for the demonstration of placental cytotrophoblast cells is described, utilising the affinity of the lectin from Bandeiraea simplicifolia-II (BSA-II) for intracellular amylase-sensitive glycogen and a protocol using biotinylated BSA-II followed by an avidin-peroxidase revealing system. In early pregnancy, cytotrophoblast cells in chorionic and anchoring villi are deeply stained and with ongoing differentiation the staining gradually decreases in intensity, suggesting that this lectin can be a useful marker for these cells. © 2016 Elsevier Ltd. All rights reserved.

Original language	Undefined
Pages (from-to)	84-86
Number of pages	3
Journal	Placenta
Volume	42
DOIs	https://doi.org/10.1016/j.placenta.2016.03.014
Publication status	Published - 2016

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10.1016/j.placenta.2016.03.014

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Cite this

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title = "A simple histochemical method for the identification of cytotrophoblasts in tissue sections",

abstract = "A simple method for the demonstration of placental cytotrophoblast cells is described, utilising the affinity of the lectin from Bandeiraea simplicifolia-II (BSA-II) for intracellular amylase-sensitive glycogen and a protocol using biotinylated BSA-II followed by an avidin-peroxidase revealing system. In early pregnancy, cytotrophoblast cells in chorionic and anchoring villi are deeply stained and with ongoing differentiation the staining gradually decreases in intensity, suggesting that this lectin can be a useful marker for these cells. {\textcopyright} 2016 Elsevier Ltd. All rights reserved.",

author = "Carolyn Jones and Chris Whittle and John Aplin",

note = "cited By 0",

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journal = "Placenta",

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T1 - A simple histochemical method for the identification of cytotrophoblasts in tissue sections

AU - Jones, Carolyn

AU - Whittle, Chris

AU - Aplin, John

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N2 - A simple method for the demonstration of placental cytotrophoblast cells is described, utilising the affinity of the lectin from Bandeiraea simplicifolia-II (BSA-II) for intracellular amylase-sensitive glycogen and a protocol using biotinylated BSA-II followed by an avidin-peroxidase revealing system. In early pregnancy, cytotrophoblast cells in chorionic and anchoring villi are deeply stained and with ongoing differentiation the staining gradually decreases in intensity, suggesting that this lectin can be a useful marker for these cells. © 2016 Elsevier Ltd. All rights reserved.

AB - A simple method for the demonstration of placental cytotrophoblast cells is described, utilising the affinity of the lectin from Bandeiraea simplicifolia-II (BSA-II) for intracellular amylase-sensitive glycogen and a protocol using biotinylated BSA-II followed by an avidin-peroxidase revealing system. In early pregnancy, cytotrophoblast cells in chorionic and anchoring villi are deeply stained and with ongoing differentiation the staining gradually decreases in intensity, suggesting that this lectin can be a useful marker for these cells. © 2016 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.placenta.2016.03.014

DO - 10.1016/j.placenta.2016.03.014

M3 - Article

SN - 0143-4004

VL - 42

SP - 84

EP - 86

JO - Placenta

JF - Placenta

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