A Systematic, Data-driven Approach to the Combined Analysis of Microarray and QTL Data

C Rennie, H Hulme, P Fisher, L Hall, M Agaba, H A Noyes, S J Kemp, A Brass, M H Pinard (Editor), C Gay (Editor), P P Pastoret (Editor), B Dodet (Editor), European Anim Dis Biol Sci Res Council (Editor), Hlth Genom Network Excellence Anim (Editor), Inst Natl Rech Agron Food Safety (Editor), World Org Anim Hlth Int Assoc Biol (Editor)

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    High-throughput technologies inevitably produce vast quantities of data. This presents challenges in terms of developing effective analysis methods, particularly where the analysis involves combining data derived from different experimental technologies. In this investigation, a systematic approach was applied to combine microarray gene expression data, quantitative trait loci (QTL) data and pathway analysis resources in order to identify functional candidate genes underlying tolerance to Trypanosoma congolense infection in cattle. We automated much of the analysis using Taverna workflows previously developed for the study of trypanotolerance in the mouse model. Pathways represented by genes within the QTL regions were identified, and this list was subsequently ranked according to which pathways were over-represented in the set of genes that were differentially expressed (over time or between tolerant N'dama and susceptible Boran breeds) at various timepoints after T congolense infection. The genes within the QTL that played a role in the highest ranked pathways were flagged as good targets for further investigation and experimental confirmation.
    Original languageEnglish
    Title of host publicationInternational Symposium on Animal Genomics for Animal Health
    EditorsM H Pinard, C Gay, P P Pastoret, B Dodet, European Anim Dis Biol Sci Res Council, Hlth Genom Network Excellence Anim, Inst Natl Rech Agron Food Safety, World Org Anim Hlth Int Assoc Biol
    PublisherS. Karger AG
    Pages293-299
    Number of pages7
    Volume132
    ISBN (Print)1424-6074 978-3-8055-8619-1
    Publication statusPublished - 2007
    EventInternational Symposium on Animal Genomics for Animal Health - Paris, FRANCE
    Duration: 25 Oct 200727 Oct 2007

    Conference

    ConferenceInternational Symposium on Animal Genomics for Animal Health
    CityParis, FRANCE
    Period25/10/0727/10/07

    Keywords

    • automated analysis
    • microarray
    • QTL
    • workflow
    • Biology
    • Biotechnology & Applied Microbiology

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