A Toolbox for Diverse Oxyfunctionalisation of Monoterpenes

Aitor Hernandez Ortega, Maria Vinaixa, Ziga Zebec, Eriko Takano, Nigel Scrutton

Research output: Contribution to journalArticlepeer-review


The successful implementation of synthetic biology for chemicals biosynthesis relies on the availability of large libraries of well-characterized enzymatic building blocks. Here we present a scalable pipeline that applies the methodology of synthetic biology itself to bootstrap the creation of such a library. By designing and building a cytochrome P450 enzyme collection and testing it in a custom-made untargeted GC/MS-metabolomics-based approach, we were able to rapidly create and characterize a comprehensive enzyme library for the controlled oxyfunctionalisation of terpene scaffolds with a wide range of activities and selectivities towards several monoterpenes. This novel resource can now be used to access the extensive chemical diversity of terpenoids by pathway engineering and the assembly of biocatalytic cascades to subsequently produce libraries of oxygenated terpenoids and their derivatives for diverse applications, including drug discovery.
Original languageEnglish
Article number14396
Pages (from-to)14396
Number of pages8
JournalScientific Reports
Early online date26 Sept 2018
Publication statusPublished - 2018

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology


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