Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.

Andrew P Breidenbach; Lindsey Aschbacher-Smith; Yinhui Lu; Nathaniel A Dyment; Chia-Feng Liu; Han Liu; Chris Wylie; Marepalli Rao; Jason T Shearn; David W Rowe; Karl E Kadler; Rulang Jiang; David L Butler

doi:10.1002/jor.22899

Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.

Andrew P Breidenbach, Lindsey Aschbacher-Smith, Yinhui Lu, Nathaniel A Dyment, Chia-Feng Liu, Han Liu, Chris Wylie, Marepalli Rao, Jason T Shearn, David W Rowe, Karl E Kadler, Rulang Jiang, David L Butler

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Abstract

Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, p

Original language	English
Journal	Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Volume	33
Issue number	8
DOIs	https://doi.org/10.1002/jor.22899 https://doi.org/10.1002/jor.22899
Publication status	Published - Aug 2015

Keywords

enthesis
fibrocartilage
hedgehog
mineralization
serial block face-scanning electron microscopy
tendon mechanics

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Breidenbach, A. P., Aschbacher-Smith, L., Lu, Y., Dyment, N. A., Liu, C.-F., Liu, H., Wylie, C., Rao, M., Shearn, J. T., Rowe, D. W., Kadler, K. E., Jiang, R., & Butler, D. L. (2015). Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 33(8). https://doi.org/10.1002/jor.22899, https://doi.org/10.1002/jor.22899

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title = "Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.",

abstract = "Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, p",

keywords = "enthesis, fibrocartilage, hedgehog, mineralization, serial block face-scanning electron microscopy, tendon mechanics",

author = "Breidenbach, {Andrew P} and Lindsey Aschbacher-Smith and Yinhui Lu and Dyment, {Nathaniel A} and Chia-Feng Liu and Han Liu and Chris Wylie and Marepalli Rao and Shearn, {Jason T} and Rowe, {David W} and Kadler, {Karl E} and Rulang Jiang and Butler, {David L}",

note = "091840/Z/10/Z, Wellcome Trust, United KingdomAR056943-05, NIAMS NIH HHS, United StatesDE013681, NIDCR NIH HHS, United StatesDE021989-02, NIDCR NIH HHS, United StatesR01 AR056943, NIAMS NIH HHS, United States",

year = "2015",

month = aug,

doi = "10.1002/jor.22899",

language = "English",

volume = "33",

journal = "Journal of orthopaedic research : official publication of the Orthopaedic Research Society",

issn = "1554-527X",

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Breidenbach, AP, Aschbacher-Smith, L, Lu, Y, Dyment, NA, Liu, C-F, Liu, H, Wylie, C, Rao, M, Shearn, JT, Rowe, DW, Kadler, KE, Jiang, R & Butler, DL 2015, 'Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.', Journal of orthopaedic research : official publication of the Orthopaedic Research Society, vol. 33, no. 8. https://doi.org/10.1002/jor.22899, https://doi.org/10.1002/jor.22899

Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. / Breidenbach, Andrew P; Aschbacher-Smith, Lindsey; Lu, Yinhui et al.
In: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 33, No. 8, 08.2015.

Research output: Contribution to journal › Article › peer-review

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AU - Aschbacher-Smith, Lindsey

AU - Lu, Yinhui

AU - Dyment, Nathaniel A

AU - Liu, Chia-Feng

AU - Liu, Han

AU - Wylie, Chris

AU - Rao, Marepalli

AU - Shearn, Jason T

AU - Rowe, David W

AU - Kadler, Karl E

AU - Jiang, Rulang

AU - Butler, David L

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AB - Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, p

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KW - fibrocartilage

KW - hedgehog

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SN - 1554-527X

VL - 33

JO - Journal of orthopaedic research : official publication of the Orthopaedic Research Society

JF - Journal of orthopaedic research : official publication of the Orthopaedic Research Society

IS - 8

ER -

Breidenbach AP, Aschbacher-Smith L, Lu Y, Dyment NA, Liu CF, Liu H et al. Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2015 Aug;33(8). doi: 10.1002/jor.22899, 10.1002/jor.22899

Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.

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Keywords

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