Absolute quantification of uric acid in human urine using surface enhanced Raman scattering with the standard addition method

Chloe Westley; Yunji Xu; Baskaran Thilaganathan; Andrew J.  Carnell; Nicholas Turner; Royston Goodacre

doi:10.1021/acs.analchem.6b04588

Absolute quantification of uric acid in human urine using surface enhanced Raman scattering with the standard addition method

Chloe Westley, Yunji Xu, Baskaran Thilaganathan, Andrew J. Carnell, Nicholas Turner, Royston Goodacre

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Abstract

High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preeclampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreeclampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.

Original language	English
Pages (from-to)	2472–2477
Number of pages	6
Journal	Analytical Chemistry
Volume	89
Issue number	4
Early online date	19 Jan 2017
DOIs	https://doi.org/10.1021/acs.analchem.6b04588
Publication status	Published - 21 Feb 2017

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title = "Absolute quantification of uric acid in human urine using surface enhanced Raman scattering with the standard addition method",

abstract = "High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preeclampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreeclampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.",

author = "Chloe Westley and Yunji Xu and Baskaran Thilaganathan and Carnell, {Andrew J.} and Nicholas Turner and Royston Goodacre",

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T1 - Absolute quantification of uric acid in human urine using surface enhanced Raman scattering with the standard addition method

AU - Westley, Chloe

AU - Xu, Yunji

AU - Thilaganathan, Baskaran

AU - Carnell, Andrew J.

AU - Turner, Nicholas

AU - Goodacre, Royston

PY - 2017/2/21

Y1 - 2017/2/21

N2 - High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preeclampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreeclampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.

AB - High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preeclampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreeclampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.

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DO - 10.1021/acs.analchem.6b04588

M3 - Article

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JF - Analytical Chemistry

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Absolute quantification of uric acid in human urine using surface enhanced Raman scattering with the standard addition method

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