Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes

S. D. Buckingham; A. M. Hosie; R. L. Roush; D. B. Sattelle

Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes

S. D. Buckingham, A. M. Hosie, R. L. Roush, D. B. Sattelle

Research output: Contribution to journal › Article › peer-review

Abstract

Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to ECl- and were insensitive to 1.0 × 10-4 M bicuculline methiodide. An order of potency of GABA ∼ muscimol ∼ TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4′-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 × 10-5 M). © 1994.

Original language	English
Pages (from-to)	137-140
Number of pages	3
Journal	Neuroscience letters
Volume	181
Issue number	1-2
Publication status	Published - 7 Nov 1994

Keywords

Convulsant antagonist
Dieldrin resistance locus
Drosophila melanogaster
GABA receptor agonist
Receptor gene expression

Cite this

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title = "Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes",

abstract = "Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to ECl- and were insensitive to 1.0 × 10-4 M bicuculline methiodide. An order of potency of GABA ∼ muscimol ∼ TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4′-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 × 10-5 M). {\textcopyright} 1994.",

keywords = "Convulsant antagonist, Dieldrin resistance locus, Drosophila melanogaster, GABA receptor agonist, Receptor gene expression",

author = "Buckingham, {S. D.} and Hosie, {A. M.} and Roush, {R. L.} and Sattelle, {D. B.}",

year = "1994",

month = nov,

day = "7",

language = "English",

volume = "181",

pages = "137--140",

journal = "Neuroscience letters",

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TY - JOUR

T1 - Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes

AU - Buckingham, S. D.

AU - Hosie, A. M.

AU - Roush, R. L.

AU - Sattelle, D. B.

PY - 1994/11/7

Y1 - 1994/11/7

N2 - Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to ECl- and were insensitive to 1.0 × 10-4 M bicuculline methiodide. An order of potency of GABA ∼ muscimol ∼ TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4′-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 × 10-5 M). © 1994.

AB - Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to ECl- and were insensitive to 1.0 × 10-4 M bicuculline methiodide. An order of potency of GABA ∼ muscimol ∼ TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4′-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 × 10-5 M). © 1994.

KW - Convulsant antagonist

KW - Dieldrin resistance locus

KW - Drosophila melanogaster

KW - GABA receptor agonist

KW - Receptor gene expression

M3 - Article

C2 - 7898754

SN - 0304-3940

VL - 181

SP - 137

EP - 140

JO - Neuroscience letters

JF - Neuroscience letters

IS - 1-2

ER -

Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homo-oligomer expressed in Xenopus oocytes

Abstract

Keywords

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