Actions of snake neurotoxins on an insect nicotinic cholinergic synapse

Bernard Hue; Steven D. Buckingham; David Buckingham; David B. Sattelle

doi:10.1007/s10158-007-0053-3

Actions of snake neurotoxins on an insect nicotinic cholinergic synapse

Bernard Hue, Steven D. Buckingham, David Buckingham, David B. Sattelle

Research output: Contribution to journal › Article › peer-review

Abstract

Here we examine the actions of six snake neurotoxins (α-cobratoxin from Naja naja siamensis, erabutoxin-a and b from Laticauda semifasciata; CM12 from N. haje annulifera, toxin III 4 from Notechis scutatus and a long toxin from N. haje) on nicotinic acetylcholine receptors in the cercal afferent, giant interneurone 2 synapse of the cockroach, Periplaneta americana. All toxins tested reduced responses to directly-applied ACh as well as EPSPs evoked by electrical stimulation of nerve XI with similar time courses, suggesting that their action is postsynaptic. Thus, these nicotinic receptors in a well-characterized insect synapse are senstive to both long and short chain neurotoxins. This considerably expands the range of snake toxins that block insect nicotinic acetylcholine receptors and may enable further pharmacological distinctions between nAChR subtypes. © 2007 Springer-Verlag.

Original language	English
Pages (from-to)	173-178
Number of pages	5
Journal	Invertebrate Neuroscience
Volume	7
Issue number	3
DOIs	https://doi.org/10.1007/s10158-007-0053-3
Publication status	Published - Sept 2007

Keywords

Insect central nervous system
Nicotinic acetylcholine receptors
Snake neurotoxins

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10.1007/s10158-007-0053-3

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title = "Actions of snake neurotoxins on an insect nicotinic cholinergic synapse",

abstract = "Here we examine the actions of six snake neurotoxins (α-cobratoxin from Naja naja siamensis, erabutoxin-a and b from Laticauda semifasciata; CM12 from N. haje annulifera, toxin III 4 from Notechis scutatus and a long toxin from N. haje) on nicotinic acetylcholine receptors in the cercal afferent, giant interneurone 2 synapse of the cockroach, Periplaneta americana. All toxins tested reduced responses to directly-applied ACh as well as EPSPs evoked by electrical stimulation of nerve XI with similar time courses, suggesting that their action is postsynaptic. Thus, these nicotinic receptors in a well-characterized insect synapse are senstive to both long and short chain neurotoxins. This considerably expands the range of snake toxins that block insect nicotinic acetylcholine receptors and may enable further pharmacological distinctions between nAChR subtypes. {\textcopyright} 2007 Springer-Verlag.",

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T1 - Actions of snake neurotoxins on an insect nicotinic cholinergic synapse

AU - Hue, Bernard

AU - Buckingham, Steven D.

AU - Buckingham, David

AU - Sattelle, David B.

PY - 2007/9

Y1 - 2007/9

N2 - Here we examine the actions of six snake neurotoxins (α-cobratoxin from Naja naja siamensis, erabutoxin-a and b from Laticauda semifasciata; CM12 from N. haje annulifera, toxin III 4 from Notechis scutatus and a long toxin from N. haje) on nicotinic acetylcholine receptors in the cercal afferent, giant interneurone 2 synapse of the cockroach, Periplaneta americana. All toxins tested reduced responses to directly-applied ACh as well as EPSPs evoked by electrical stimulation of nerve XI with similar time courses, suggesting that their action is postsynaptic. Thus, these nicotinic receptors in a well-characterized insect synapse are senstive to both long and short chain neurotoxins. This considerably expands the range of snake toxins that block insect nicotinic acetylcholine receptors and may enable further pharmacological distinctions between nAChR subtypes. © 2007 Springer-Verlag.

AB - Here we examine the actions of six snake neurotoxins (α-cobratoxin from Naja naja siamensis, erabutoxin-a and b from Laticauda semifasciata; CM12 from N. haje annulifera, toxin III 4 from Notechis scutatus and a long toxin from N. haje) on nicotinic acetylcholine receptors in the cercal afferent, giant interneurone 2 synapse of the cockroach, Periplaneta americana. All toxins tested reduced responses to directly-applied ACh as well as EPSPs evoked by electrical stimulation of nerve XI with similar time courses, suggesting that their action is postsynaptic. Thus, these nicotinic receptors in a well-characterized insect synapse are senstive to both long and short chain neurotoxins. This considerably expands the range of snake toxins that block insect nicotinic acetylcholine receptors and may enable further pharmacological distinctions between nAChR subtypes. © 2007 Springer-Verlag.

KW - Insect central nervous system

KW - Nicotinic acetylcholine receptors

KW - Snake neurotoxins

U2 - 10.1007/s10158-007-0053-3

DO - 10.1007/s10158-007-0053-3

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JO - Invertebrate Neuroscience

JF - Invertebrate Neuroscience

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Actions of snake neurotoxins on an insect nicotinic cholinergic synapse

Abstract

Keywords

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