Activator-mediated disruption of sequence-specific DNA contacts by the general transcription factor TFIIB

Rachel Evans, Jennifer A. Fairley, Stefan G E Roberts

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The transcription factor TFIIB plays a central role in preinitiation complex assembly, providing a bridge between promoter-bound TFIID and RNA Polymerase II. TFIIB possesses sequence-specific DNA-binding ability and interacts with the TFIIB-recognition element (BRE), present in many promoters. Here we show that the BRE suppresses the basal level of transcription elicited by a core promoter, which increases the amplitude of transcriptional stimulation in the presence of an activator protein. Further, we find that an activator can disrupt the TFIIB-BRE interaction within a promoter-bound complex. Our results reveal a novel function for activators in the modulation of core promoter recognition by TFIIB.
    Original languageEnglish
    Pages (from-to)2945-2949
    Number of pages4
    JournalGenes and development
    Volume15
    Issue number22
    DOIs
    Publication statusPublished - 15 Nov 2001

    Keywords

    • Promoter
    • RNA polymerase II
    • TFIIB
    • Transactivation
    • Transcription

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