Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta

P. F. Speake; K. J. Mynett; J. D. Glazier; S. L. Greenwood; C. P. Sibley

doi:10.1007/s00424-005-1382-x

Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta

P. F. Speake, K. J. Mynett, J. D. Glazier, S. L. Greenwood, C. P. Sibley

Research output: Contribution to journal › Article › peer-review

Abstract

The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was four- to fivefold higher than that by BM vesicles (214±28 vs. 49±9 pmol/mg protein per 30 s, respectively, means±SEM, n=8, 6, P

Original language	English
Pages (from-to)	123-130
Number of pages	7
Journal	Pflügers Archiv European Journal of Physiology
Volume	450
Issue number	2
DOIs	https://doi.org/10.1007/s00424-005-1382-x
Publication status	Published - May 2005

Keywords

Activity
Expression
NHE1
NHE2
NHE3
pH i
Placenta
Syncytiotrophoblast

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10.1007/s00424-005-1382-x

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title = "Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta",

abstract = "The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was four- to fivefold higher than that by BM vesicles (214±28 vs. 49±9 pmol/mg protein per 30 s, respectively, means±SEM, n=8, 6, P",

keywords = "Activity, Expression, NHE1, NHE2, NHE3, pH i, Placenta, Syncytiotrophoblast",

author = "Speake, {P. F.} and Mynett, {K. J.} and Glazier, {J. D.} and Greenwood, {S. L.} and Sibley, {C. P.}",

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doi = "10.1007/s00424-005-1382-x",

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T1 - Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta

AU - Speake, P. F.

AU - Mynett, K. J.

AU - Glazier, J. D.

AU - Greenwood, S. L.

AU - Sibley, C. P.

PY - 2005/5

Y1 - 2005/5

N2 - The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was four- to fivefold higher than that by BM vesicles (214±28 vs. 49±9 pmol/mg protein per 30 s, respectively, means±SEM, n=8, 6, P

AB - The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was four- to fivefold higher than that by BM vesicles (214±28 vs. 49±9 pmol/mg protein per 30 s, respectively, means±SEM, n=8, 6, P

KW - Activity

KW - Expression

KW - NHE1

KW - NHE2

KW - NHE3

KW - pH i

KW - Placenta

KW - Syncytiotrophoblast

U2 - 10.1007/s00424-005-1382-x

DO - 10.1007/s00424-005-1382-x

M3 - Article

SN - 0031-6768

VL - 450

SP - 123

EP - 130

JO - Pflügers Archiv European Journal of Physiology

JF - Pflügers Archiv European Journal of Physiology

IS - 2

ER -

Activity and expression of Na+/H+ exchanger isoforms in the syncytiotrophoblast of the human placenta

Abstract

Keywords

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