Activity of human contact allergens in the murine local lymph node assay

C. A. Ryan, G. F. Gerberick, L. W. Cruse, D. A. Basketter, L. Lea, L. Blaikie, R. J. Dearman, E. V. Warbrick, I. Kimber

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The murine local lymph node assay (LLNA) is a predictive test for the identification of chemicals that have the potential to cause skin sensitization. Since its original development, the assay has been the subject of national and international evaluation studies and extensive comparisons with guinea pig tests and human data. On the basis of these investigations, the LLNA has recently been endorsed by ICCVAM (Interagency Coordinating Committee on the Validation of Alternative Methods) as a stand-alone method for skin sensitization hazard identification. At the same time, ICCVAM confirmed that, although the LLNA is not an in vitro method, it does represent a refinement in the way animals are used and can provide a means for reducing the number of animals used in sensitization hazard assessment. The investigations described here were designed to explore further the ability of the LLNA to identify accurately those chemicals that cause allergic contact dermatitis in humans. To that end we have measured, amongst 3 independent laboratories, LLNA responses induced by a total of 18 test chemicals, 11 of which are known to cause skin sensitization and 7 of which are believed not to be associated with any significant evidence of allergic contact dermatitis in humans. The LLNA correctly classified 16 of the 18 materials. The 11 chemicals tested which are associated with allergic contact dermatitis in humans were found to be positive in the LLNA. Of the 7 materials believed to be non-sensitizers, 5 were negative in the LLNA and 2 produced positive results. Collectively, these data provide additional evidence that the LLNA is able to discriminate skin sensitizers from those chemicals which do not possess a significant skin sensitization potential and thus provides a method for hazard identification that offers important animal welfare benefits. (C) Munksgaard, 2000.
    Original languageEnglish
    Pages (from-to)95-102
    Number of pages7
    JournalContact dermatitis
    Volume43
    Issue number2
    DOIs
    Publication statusPublished - 2000

    Keywords

    • Contact allergy
    • Fragrance
    • Isopropyl myristate
    • Linalool
    • Local lymph node assay
    • Methyl 4- hydroxybenzoate
    • Mouse sensitization
    • Parabens
    • Predictive testing

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