Acute changes in MRI diffusion, perfusion, T1, and T2 in a rat model of oligemia produced by partial occlusion of the middle cerebral artery

Mark F. Lythgoe, David L. Thomas, Fernando Calamante, Gaby S. Pell, Martin D. King, Albert L. Busza, Christopher H. Sotak, Stephen R. Williams, Roger J. Ordidge, David G. Gadian

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Oligemic regions, in which the cerebral blood flow is reduced without impaired energy metabolism, have the potential to evolve toward infarction and remain a target for therapy. The aim of this study was to investigate this oligemic region using various MRI parameters in a rat model of focal oligemia. This model has been designed specifically for remote-controlled occlusion from outside an MRI scanner. Wistar rats underwent remote partial MCAO using an undersize 0.2 mm nylon monofilament with a bulletshaped tip. Cerebral blood flow (CBF(ASL)), using an arterial spin labeling technique, the apparent diffusion coefficient of water (ADC), and the relaxation times T1 and T2 were acquired using an 8.5 T vertical magnet. Following occlusion there was a decrease in CBF(ASL) to 35 ± 5% of baseline throughout the middle cerebral artery territory. During the entire period of the study there were no observed changes in the ADC. On occlusion, T2 rapidly decreased in both cortex and basal ganglia and then normalized to the preocclusion values. T1 values rapidly increased (within approximately 7 min) on occlusion. In conclusion, this study demonstrates the feasibility of partially occluding the middle cerebral artery to produce a large area of oligemia within the MRI scanner. In this region of oligemic flow we detect a rapid increase in T1 and decrease in T2. These changes occur before the onset of vasogenic edema. We attribute the acute change in T2 to increased amounts of deoxyhemoglobin; the mechanisms underlying the change in T1 require further investigation. (C) 2000 Wiley-Liss, Inc.
    Original languageEnglish
    Pages (from-to)706-712
    Number of pages6
    JournalMagnetic Resonance in Medicine
    Volume44
    Issue number5
    DOIs
    Publication statusPublished - 2000

    Keywords

    • Cerebral ischemia
    • Magnetic resonance imaging
    • Middle cerebral artery occlusion
    • Penumbra

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