Adenosine triphosphate in exhaled breath condensate of healthy subjects and patients with chronic obstructive pulmonary disease

Z. Lázár; É. Huszár; T. Kullmann; I. Barta; B. Antus; A. Bikov; M. Kollai; I. Horváth

doi:10.1007/s00011-008-8009-6

Adenosine triphosphate in exhaled breath condensate of healthy subjects and patients with chronic obstructive pulmonary disease

Z. Lázár, É. Huszár, T. Kullmann, I. Barta, B. Antus, A. Bikov, M. Kollai, I. Horváth

Division of Immunology, Immunity to Infection and Respiratory Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: The effect of hypoxic relapse of chronic obstructive pulmonary disease (COPD) on lung adenosine triphosphate (ATP) concentration was studied measuring ATP in exhaled breath condensate (EBC). Subjects: Thirty COPD patients with severe exacerbation, thirteen healthy non-smokers and thirteen healthy smokers. Methods: ATP was detected using a luciferin-luciferase assay, dilution of airway droplets in EBC was assessed measuring sample conductivity. Results: ATP concentrations were similar in COPD patients, non-smoking and smoking healthy individuals (141 ± 44, 115 ± 21 and 90 ± 15 pM; p = 0.66). After treatment oxygenation of COPD patients improved (6.85 ± 1.29 kPa vs 8.20 ± 1.28 kPa, p

Original language	English
Pages (from-to)	367-373
Number of pages	7
Journal	Inflammation Research
Volume	57
Issue number	8
DOIs	https://doi.org/10.1007/s00011-008-8009-6
Publication status	Published - 2008

Keywords

Adenosine triphosphate
Airways
Chronic obstructive pulmonary disease
Exhaled breath condensate
Hypoxia
adenosine triphosphate
luciferase
luciferin
adult
article
chronic obstructive lung disease
clinical article
controlled study
disease exacerbation
disease marker
enzyme assay
exhalation
female
human
hypoxia
male
oxygenation
protein blood level
protein determination
smoking
Adenosine Triphosphate
Adult
Aged
Anoxia
Body Fluids
Breath Tests
Exhalation
Female
Humans
Male
Middle Aged
Pulmonary Disease, Chronic Obstructive
Smoking

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10.1007/s00011-008-8009-6

https://www.scopus.com/inward/record.uri?eid=2-s2.0-51749106369&doi=10.1007%2fs00011-008-8009-6&partnerID=40&md5=d7de220e7f849cb203892e6d37f2a508

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@article{5618cc0ab2cc408eb8ff6185611a4ac5,

title = "Adenosine triphosphate in exhaled breath condensate of healthy subjects and patients with chronic obstructive pulmonary disease",

abstract = "Objectives: The effect of hypoxic relapse of chronic obstructive pulmonary disease (COPD) on lung adenosine triphosphate (ATP) concentration was studied measuring ATP in exhaled breath condensate (EBC). Subjects: Thirty COPD patients with severe exacerbation, thirteen healthy non-smokers and thirteen healthy smokers. Methods: ATP was detected using a luciferin-luciferase assay, dilution of airway droplets in EBC was assessed measuring sample conductivity. Results: ATP concentrations were similar in COPD patients, non-smoking and smoking healthy individuals (141 ± 44, 115 ± 21 and 90 ± 15 pM; p = 0.66). After treatment oxygenation of COPD patients improved (6.85 ± 1.29 kPa vs 8.20 ± 1.28 kPa, p ",

keywords = "Adenosine triphosphate, Airways, Chronic obstructive pulmonary disease, Exhaled breath condensate, Hypoxia, adenosine triphosphate, luciferase, luciferin, adult, article, chronic obstructive lung disease, clinical article, controlled study, disease exacerbation, disease marker, enzyme assay, exhalation, female, human, hypoxia, male, oxygenation, protein blood level, protein determination, smoking, Adenosine Triphosphate, Adult, Aged, Anoxia, Body Fluids, Breath Tests, Exhalation, Female, Humans, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive, Smoking",

author = "Z. L{\'a}z{\'a}r and {\'E}. Husz{\'a}r and T. Kullmann and I. Barta and B. Antus and A. Bikov and M. Kollai and I. Horv{\'a}th",

note = "Cited By :21 Export Date: 20 February 2019 CODEN: INREF Correspondence Address: Horv{\'a}th, I.; Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, 78/a {\"U}ll{\"o}i Street, Budapest, 1082, Hungary; email: [email protected] Chemicals/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; luciferin, 2591-17-5; Adenosine Triphosphate, 56-65-5 Funding details: 68808 Funding details: Canon Foundation for Scientific Research, CFSR, OTKA 43396 Funding text 1: Funding. This study was supported by the Hungarian Scientific Research Foundation (OTKA 43396 and 68808). References: Adriaensen, D., Timmermans, J.P., Purinergic signalling in the lung: Important in asthma and COPD? (2004) Current Opinion Pharmacol, 4, pp. 207-214; Oguma, T., Ito, S., Kondo, M., Makino, Y., Shimokata, K., Honjo, H., Roles of P2X receptors and Ca2+ sensitization in extracellular adenosine triphosphate-induced hyperresponsiveness in airway smooth muscle (2007) Clin Exp Allergy, 37, pp. 893-900; Pelleg, A., Hurt, C.M., Mechanism of action of ATP on canine pulmonary vagal C fibre nerve terminals (1996) J Physiol, 490, pp. 265-275; Idzko, M., Panther, E., Bremer, H.C., Sorichter, S., Luttmann, W., Virchow Jr., C.J., Stimulation of P2 purinergic receptors induces the release of eosinophil cationic protein and interleukin-8 from human eosinophils (2003) Br J Pharmacol, 138, pp. 1244-1250; Idzko, M., Hammad, H., van Nimwegen, M., Kool, M., Willart, M.A., Muskens, F., Extracellular ATP triggers and maintains asthmatic airway inflammation by activating dendritic cells (2007) Nat Med, 13, pp. 913-919; Bodin, P., Burnstock, G., Increased release of ATP from endothelial cells during acute inflammation (1998) Inflamm Res, 47, pp. 351-354; Pelleg, A., Schulman, E.S., Adenosine 5′-triphosphate axis in obstructive airway diseases (2002) Am J Ther, 9, pp. 454-464; Gerasimovskaya, E.V., Ahmad, S., White, C.W., Jones, P.L., Carpenter, T.C., Stenmark, K.R., Extracellular ATP is an autocrine/paracrine regulator of hypoxia-induced adventitial fibroblast growth. Signaling through extracellular signal-regulated kinase-1/2 and the Egr-1 transcription factor (2002) J Biol Chem, 277, pp. 44638-44650; Burnstock, G., Pathophysiology and therapeutic potential of purinergic signaling (2006) Pharmacol Rev, 58, pp. 58-86; Papen, M., Wodopia, R., Bartsch, P., Mairbaurl, H., Hypoxia-effects on Ca(i)-signaling and ion transport activity of lung alveolar epithelial cells (2001) Cell Physiol Biochem, 11, pp. 187-196; Donaldson, S.H., Lazarowski, E.R., Picher, M., Knowles, M.R., Stutts, M.J., Boucher, R.C., Basal nucleotide levels, release, and metabolism in normal and cystic fibrosis airways (2000) Mol Med, 6, pp. 969-982; Grygorczyk, R., Hanrahan, J.W., CFTR-independent ATP release from epithelial cells triggered by mechanical stimuli (1997) Am J Physiol, 272, pp. C1058-C1066; Watt, W.C., Lazarowski, E.R., Boucher, R.C., Cystic fibrosis transmembrane regulator-independent release of ATP. Its implications for the regulation of P2Y2 receptors in airway epithelia (1998) J Biol Chem, 273, pp. 14053-14058; Huszar, E., Szabo, Z., Jakab, A., Barta, I., Herjavecz, I., Horvath, I., Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays (2005) Inflamm Res, 54, pp. 350-355; Montuschi, P., Ragazzoni, E., Valente, S., Corbo, G., Mondino, C., Ciappi, G., Validation of leukotriene B4 measurements in exhaled breath condensate (2003) Inflamm Res, 52, pp. 69-73; Horvath, I., Hunt, J., Barnes, P.J., Alving, K., Antczak, A., Baraldi, E., Exhaled breath condensate: Methodological recommendations and unresolved questions (2005) Eur Respir J, 26, pp. 523-548; Rabe, K.F., Hurd, S., Anzueto, A., Barnes, P.J., Buist, S.A., Calverley, P., Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary (2007) Am J Resp Crit Care Med, 176, pp. 532-555; Miller, M.R., Hankinson, J., Brusasco, V., Burgos, F., Casaburi, R., Coates, A., Standardisation of spirometry (2005) Eur Respir J, 26, pp. 319-338; Kharitonov, S., Alving, K., Barnes, P.J., Exhaled and nasal nitric oxide measurements: Recommendations. The European Respiratory Society Task Force (1997) Eur Respir J, 10, pp. 1683-1693; Bland, J.M., Altman, D.G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 1, pp. 307-310; Buchner, A., Erdfelder, E., Faul, F., (1997) How to Use G*Power [WWW document], , http://www.psycho.uni-duesseldorf.de/aap/projects/gpower/how_to_use_gpower.html, URL; Effros, R.M., Peterson, B., Casaburi, R., Su, J., Dunning, M., Torday, J., Epithelial lining fluid solute concentrations in chronic obstructive lung disease patients and normal subjects (2005) J Appl Physiol, 99, pp. 1286-1292; Picher, M., Burch, L.H., Boucher, R.C., Metabolism of P2 receptor agonists in human airways: Implications for mucociliary clearance and cystic fibrosis (2004) J Biol Chem, 279, pp. 20234-20241; Gessner, C., Scheibe, R., Wotzel, M., Hammerschmidt, S., Kuhn, H., Engelmann, L., Exhaled breath condensate cytokine patterns in chronic obstructive pulmonary disease (2005) Respir Med, 99, pp. 1229-1240; Dwyer, T.M., Sampling airway surface liquid: Non-volatiles in the exhaled breath condensate (2004) Lung, 182, pp. 241-250; Csoma, Z., Huszar, E., Vizi, E., Vass, G., Szabo, Z., Herjavecz, I., Adenosine level in exhaled breath increases during exercise-induced bronchoconstriction (2005) Eur Respir J, 25, pp. 873-878; Huszar, E., Vass, G., Vizi, E., Csoma, Z., Barat, E., Molnar Vilagos, G., Adenosine in exhaled breath condensate in healthy volunteers and in patients with asthma (2002) Eur Respir J, 20, pp. 1393-1398; Vass, G., Huszar, E., Barat, E., Valyon, M., Kiss, D., Penzes, I., Comparison of nasal and oral inhalation during exhaled breath condensate collection (2003) Am J Resp Crit Care Med, 167, pp. 850-855; Vass, G., Horvath, I., Adenosine and adenosine receptors in the pathomechanism and treatment of respiratory diseases (2008) Current Med Chem, 15, pp. 917-922",

year = "2008",

doi = "10.1007/s00011-008-8009-6",

language = "English",

volume = "57",

pages = "367--373",

journal = "Inflammation Research",

issn = "1023-3830",

publisher = "Birkh{\"a}user Verlag Ag",

number = "8",

}

TY - JOUR

T1 - Adenosine triphosphate in exhaled breath condensate of healthy subjects and patients with chronic obstructive pulmonary disease

AU - Lázár, Z.

AU - Huszár, É.

AU - Kullmann, T.

AU - Barta, I.

AU - Antus, B.

AU - Bikov, A.

AU - Kollai, M.

AU - Horváth, I.

N1 - Cited By :21 Export Date: 20 February 2019 CODEN: INREF Correspondence Address: Horváth, I.; Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, 78/a Üllöi Street, Budapest, 1082, Hungary; email: [email protected] Chemicals/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; luciferin, 2591-17-5; Adenosine Triphosphate, 56-65-5 Funding details: 68808 Funding details: Canon Foundation for Scientific Research, CFSR, OTKA 43396 Funding text 1: Funding. This study was supported by the Hungarian Scientific Research Foundation (OTKA 43396 and 68808). References: Adriaensen, D., Timmermans, J.P., Purinergic signalling in the lung: Important in asthma and COPD? (2004) Current Opinion Pharmacol, 4, pp. 207-214; Oguma, T., Ito, S., Kondo, M., Makino, Y., Shimokata, K., Honjo, H., Roles of P2X receptors and Ca2+ sensitization in extracellular adenosine triphosphate-induced hyperresponsiveness in airway smooth muscle (2007) Clin Exp Allergy, 37, pp. 893-900; Pelleg, A., Hurt, C.M., Mechanism of action of ATP on canine pulmonary vagal C fibre nerve terminals (1996) J Physiol, 490, pp. 265-275; Idzko, M., Panther, E., Bremer, H.C., Sorichter, S., Luttmann, W., Virchow Jr., C.J., Stimulation of P2 purinergic receptors induces the release of eosinophil cationic protein and interleukin-8 from human eosinophils (2003) Br J Pharmacol, 138, pp. 1244-1250; Idzko, M., Hammad, H., van Nimwegen, M., Kool, M., Willart, M.A., Muskens, F., Extracellular ATP triggers and maintains asthmatic airway inflammation by activating dendritic cells (2007) Nat Med, 13, pp. 913-919; Bodin, P., Burnstock, G., Increased release of ATP from endothelial cells during acute inflammation (1998) Inflamm Res, 47, pp. 351-354; Pelleg, A., Schulman, E.S., Adenosine 5′-triphosphate axis in obstructive airway diseases (2002) Am J Ther, 9, pp. 454-464; Gerasimovskaya, E.V., Ahmad, S., White, C.W., Jones, P.L., Carpenter, T.C., Stenmark, K.R., Extracellular ATP is an autocrine/paracrine regulator of hypoxia-induced adventitial fibroblast growth. Signaling through extracellular signal-regulated kinase-1/2 and the Egr-1 transcription factor (2002) J Biol Chem, 277, pp. 44638-44650; Burnstock, G., Pathophysiology and therapeutic potential of purinergic signaling (2006) Pharmacol Rev, 58, pp. 58-86; Papen, M., Wodopia, R., Bartsch, P., Mairbaurl, H., Hypoxia-effects on Ca(i)-signaling and ion transport activity of lung alveolar epithelial cells (2001) Cell Physiol Biochem, 11, pp. 187-196; Donaldson, S.H., Lazarowski, E.R., Picher, M., Knowles, M.R., Stutts, M.J., Boucher, R.C., Basal nucleotide levels, release, and metabolism in normal and cystic fibrosis airways (2000) Mol Med, 6, pp. 969-982; Grygorczyk, R., Hanrahan, J.W., CFTR-independent ATP release from epithelial cells triggered by mechanical stimuli (1997) Am J Physiol, 272, pp. C1058-C1066; Watt, W.C., Lazarowski, E.R., Boucher, R.C., Cystic fibrosis transmembrane regulator-independent release of ATP. Its implications for the regulation of P2Y2 receptors in airway epithelia (1998) J Biol Chem, 273, pp. 14053-14058; Huszar, E., Szabo, Z., Jakab, A., Barta, I., Herjavecz, I., Horvath, I., Comparative measurement of thromboxane A2 metabolites in exhaled breath condensate by different immunoassays (2005) Inflamm Res, 54, pp. 350-355; Montuschi, P., Ragazzoni, E., Valente, S., Corbo, G., Mondino, C., Ciappi, G., Validation of leukotriene B4 measurements in exhaled breath condensate (2003) Inflamm Res, 52, pp. 69-73; Horvath, I., Hunt, J., Barnes, P.J., Alving, K., Antczak, A., Baraldi, E., Exhaled breath condensate: Methodological recommendations and unresolved questions (2005) Eur Respir J, 26, pp. 523-548; Rabe, K.F., Hurd, S., Anzueto, A., Barnes, P.J., Buist, S.A., Calverley, P., Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary (2007) Am J Resp Crit Care Med, 176, pp. 532-555; Miller, M.R., Hankinson, J., Brusasco, V., Burgos, F., Casaburi, R., Coates, A., Standardisation of spirometry (2005) Eur Respir J, 26, pp. 319-338; Kharitonov, S., Alving, K., Barnes, P.J., Exhaled and nasal nitric oxide measurements: Recommendations. The European Respiratory Society Task Force (1997) Eur Respir J, 10, pp. 1683-1693; Bland, J.M., Altman, D.G., Statistical methods for assessing agreement between two methods of clinical measurement (1986) Lancet, 1, pp. 307-310; Buchner, A., Erdfelder, E., Faul, F., (1997) How to Use G*Power [WWW document], , http://www.psycho.uni-duesseldorf.de/aap/projects/gpower/how_to_use_gpower.html, URL; Effros, R.M., Peterson, B., Casaburi, R., Su, J., Dunning, M., Torday, J., Epithelial lining fluid solute concentrations in chronic obstructive lung disease patients and normal subjects (2005) J Appl Physiol, 99, pp. 1286-1292; Picher, M., Burch, L.H., Boucher, R.C., Metabolism of P2 receptor agonists in human airways: Implications for mucociliary clearance and cystic fibrosis (2004) J Biol Chem, 279, pp. 20234-20241; Gessner, C., Scheibe, R., Wotzel, M., Hammerschmidt, S., Kuhn, H., Engelmann, L., Exhaled breath condensate cytokine patterns in chronic obstructive pulmonary disease (2005) Respir Med, 99, pp. 1229-1240; Dwyer, T.M., Sampling airway surface liquid: Non-volatiles in the exhaled breath condensate (2004) Lung, 182, pp. 241-250; Csoma, Z., Huszar, E., Vizi, E., Vass, G., Szabo, Z., Herjavecz, I., Adenosine level in exhaled breath increases during exercise-induced bronchoconstriction (2005) Eur Respir J, 25, pp. 873-878; Huszar, E., Vass, G., Vizi, E., Csoma, Z., Barat, E., Molnar Vilagos, G., Adenosine in exhaled breath condensate in healthy volunteers and in patients with asthma (2002) Eur Respir J, 20, pp. 1393-1398; Vass, G., Huszar, E., Barat, E., Valyon, M., Kiss, D., Penzes, I., Comparison of nasal and oral inhalation during exhaled breath condensate collection (2003) Am J Resp Crit Care Med, 167, pp. 850-855; Vass, G., Horvath, I., Adenosine and adenosine receptors in the pathomechanism and treatment of respiratory diseases (2008) Current Med Chem, 15, pp. 917-922

PY - 2008

Y1 - 2008

N2 - Objectives: The effect of hypoxic relapse of chronic obstructive pulmonary disease (COPD) on lung adenosine triphosphate (ATP) concentration was studied measuring ATP in exhaled breath condensate (EBC). Subjects: Thirty COPD patients with severe exacerbation, thirteen healthy non-smokers and thirteen healthy smokers. Methods: ATP was detected using a luciferin-luciferase assay, dilution of airway droplets in EBC was assessed measuring sample conductivity. Results: ATP concentrations were similar in COPD patients, non-smoking and smoking healthy individuals (141 ± 44, 115 ± 21 and 90 ± 15 pM; p = 0.66). After treatment oxygenation of COPD patients improved (6.85 ± 1.29 kPa vs 8.20 ± 1.28 kPa, p

AB - Objectives: The effect of hypoxic relapse of chronic obstructive pulmonary disease (COPD) on lung adenosine triphosphate (ATP) concentration was studied measuring ATP in exhaled breath condensate (EBC). Subjects: Thirty COPD patients with severe exacerbation, thirteen healthy non-smokers and thirteen healthy smokers. Methods: ATP was detected using a luciferin-luciferase assay, dilution of airway droplets in EBC was assessed measuring sample conductivity. Results: ATP concentrations were similar in COPD patients, non-smoking and smoking healthy individuals (141 ± 44, 115 ± 21 and 90 ± 15 pM; p = 0.66). After treatment oxygenation of COPD patients improved (6.85 ± 1.29 kPa vs 8.20 ± 1.28 kPa, p

KW - Adenosine triphosphate

KW - Airways

KW - Chronic obstructive pulmonary disease

KW - Exhaled breath condensate

KW - Hypoxia

KW - adenosine triphosphate

KW - luciferase

KW - luciferin

KW - adult

KW - article

KW - chronic obstructive lung disease

KW - clinical article

KW - controlled study

KW - disease exacerbation

KW - disease marker

KW - enzyme assay

KW - exhalation

KW - female

KW - human

KW - hypoxia

KW - male

KW - oxygenation

KW - protein blood level

KW - protein determination

KW - smoking

KW - Adenosine Triphosphate

KW - Adult

KW - Aged

KW - Anoxia

KW - Body Fluids

KW - Breath Tests

KW - Exhalation

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Pulmonary Disease, Chronic Obstructive

KW - Smoking

U2 - 10.1007/s00011-008-8009-6

DO - 10.1007/s00011-008-8009-6

M3 - Article

SN - 1023-3830

VL - 57

SP - 367

EP - 373

JO - Inflammation Research

JF - Inflammation Research

IS - 8

ER -

Adenosine triphosphate in exhaled breath condensate of healthy subjects and patients with chronic obstructive pulmonary disease

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