Advancing Solutions to the Carbohydrate Sequencing Challenge

Christopher J. Gray, Lukasz G. Migas, Perdita E. Barran, Kevin Pagel, Peter H Seeberger, Claire E. Eyers, Geert-jan Boons, Nicola L. B. Pohl, Isabelle Compagnon, Göran Widmalm, Sabine L. Flitsch

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Abstract

Carbohydrates possess a variety of distinct features with stereochemistry playing a particularly important role in distinguishing their structure and function. Monosaccharide building blocks are defined by a high density of chiral centers. Additionally, the anomericity and regio-chemistry of the glycosidic linkages carry important biological information. Any carbohydrate-sequencing method needs to be precise in determining all aspects of this stereo-diversity. Recently, several advances have been made in developing fast and precise analytical techniques that have the potential to address the stereochemical complexity of carbohydrates. This perspective seeks to provide an overview of some of these emerging techniques, focusing on those that are based on NMR and MS-hybridized technologies including ion mobility spectrometry and IR spectroscopy.
Original languageEnglish
JournalJournal of the American Chemical Society
Early online date12 Aug 2019
DOIs
Publication statusPublished - 2019

Keywords

  • Glycan
  • Carbohydrate
  • NMR
  • mass spectrometry
  • liquid chromatography
  • ion-mobility spectrometry
  • infrared spectroscopy

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