AFM study of cytochrome CYP102A1 oligomeric state

Yuri D. Ivanov, Natalia S. Bukharina, Pavel A. Frantsuzov, Tatyana O. Pleshakova, Sergey L. Kanashenko, Natalia V. Medvedeva, Viktoriya V. Argentova, Viktor G. Zgoda, Andrew W. Munro, Alexander I. Archakov

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Atomic force microscopy (AFM) study of the oligomeric state of CYP102A1 was carried out in three different environmental conditions - liquid, air and vacuum. It was shown that usage of a standard probe with a radius of curvature of 10 nm allowed determination of the monomers-to-oligomers ratio, α ≈ 0.5:0.5, in these three media, but it wasn't possible to estimate the oligomerization degree more precisely. Application of a supersharp probe with a radius of curvature of 2 nm made it possible not only to obtain data on the ratio of monomers-to-oligomers but also to obtain the approximate ratio between dimers, trimers and tetramers 0.3:0.1:0.1, but only in vacuum. © 2012 The Royal Society of Chemistry.
    Original languageEnglish
    Pages (from-to)4602-4608
    Number of pages6
    JournalSoft Matter
    Volume8
    Issue number17
    DOIs
    Publication statusPublished - 7 May 2012

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