Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction

S. J. Stocks; R. M. Agius; N. Cooley; K. L. Harrison; D. R. Brison; G. Horne; A. Gibbs; A. C. Povey

doi:10.1016/j.mrgentox.2010.02.019

Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction

S. J. Stocks, R. M. Agius, N. Cooley, K. L. Harrison, D. R. Brison, G. Horne, A. Gibbs, A. C. Povey

Research output: Contribution to journal › Article › peer-review

Abstract

Approximately one-third of IVF cases in the UK are attributed to male factor infertility and in the majority of cases the origin of male infertility is unknown. The integrity of sperm DNA is important both for the success of assisted reproduction and the implications for the off-spring. One type of DNA damage that has not been investigated with respect to fertility outcomes is the adduct N7-methyldeoxyguanosine (N7-MedG), a biomarker for exposure to alkylating agents. A prospective cohort of couples attending for IVF had their N7-MedG levels in sperm measured using an immunoslot blot technique to examine whether sperm N7-MedG levels are associated with male factor infertility, semen quality measures or assisted reproduction outcomes. Sufficient DNA for analysis was obtained from 67/97 couples and N7-MedG was detected in 94% of sperm samples analysed. Men diagnosed with male factor infertility had significantly higher mean levels of N7-MedG in their sperm DNA (P=. 0.03). Logistic regression analysis showed that N7-MedG levels were significantly negatively associated with the proportion of oocytes successfully fertilised irrespective of the method of fertilisation used (IVF or intra-cytoplasmic sperm injection; ICSI, P

Original language	English
Pages (from-to)	18-23
Number of pages	5
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	698
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mrgentox.2010.02.019
Publication status	Published - 30 Apr 2010

Keywords

Alkylation
DNA adducts
DNA damage
IVF
Male infertility
N7-methyldeoxyguanosine

Access to Document

10.1016/j.mrgentox.2010.02.019

Cite this

Stocks, S. J., Agius, R. M., Cooley, N., Harrison, K. L., Brison, D. R., Horne, G., Gibbs, A., & Povey, A. C. (2010). Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 698(1-2), 18-23. https://doi.org/10.1016/j.mrgentox.2010.02.019

@article{6ca69a2f8a9e483e8c3118acf0e625b9,

title = "Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction",

abstract = "Approximately one-third of IVF cases in the UK are attributed to male factor infertility and in the majority of cases the origin of male infertility is unknown. The integrity of sperm DNA is important both for the success of assisted reproduction and the implications for the off-spring. One type of DNA damage that has not been investigated with respect to fertility outcomes is the adduct N7-methyldeoxyguanosine (N7-MedG), a biomarker for exposure to alkylating agents. A prospective cohort of couples attending for IVF had their N7-MedG levels in sperm measured using an immunoslot blot technique to examine whether sperm N7-MedG levels are associated with male factor infertility, semen quality measures or assisted reproduction outcomes. Sufficient DNA for analysis was obtained from 67/97 couples and N7-MedG was detected in 94% of sperm samples analysed. Men diagnosed with male factor infertility had significantly higher mean levels of N7-MedG in their sperm DNA (P=. 0.03). Logistic regression analysis showed that N7-MedG levels were significantly negatively associated with the proportion of oocytes successfully fertilised irrespective of the method of fertilisation used (IVF or intra-cytoplasmic sperm injection; ICSI, P",

keywords = "Alkylation, DNA adducts, DNA damage, IVF, Male infertility, N7-methyldeoxyguanosine",

author = "Stocks, {S. J.} and Agius, {R. M.} and N. Cooley and Harrison, {K. L.} and Brison, {D. R.} and G. Horne and A. Gibbs and Povey, {A. C.}",

year = "2010",

month = apr,

day = "30",

doi = "10.1016/j.mrgentox.2010.02.019",

language = "English",

volume = "698",

pages = "18--23",

journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",

issn = "1383-5718",

publisher = "Elsevier BV",

number = "1-2",

}

Stocks, SJ, Agius, RM, Cooley, N, Harrison, KL, Brison, DR, Horne, G, Gibbs, A & Povey, AC 2010, 'Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction', Mutation Research - Genetic Toxicology and Environmental Mutagenesis, vol. 698, no. 1-2, pp. 18-23. https://doi.org/10.1016/j.mrgentox.2010.02.019

Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction. / Stocks, S. J.; Agius, R. M.; Cooley, N. et al.
In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 698, No. 1-2, 30.04.2010, p. 18-23.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction

AU - Stocks, S. J.

AU - Agius, R. M.

AU - Cooley, N.

AU - Harrison, K. L.

AU - Brison, D. R.

AU - Horne, G.

AU - Gibbs, A.

AU - Povey, A. C.

PY - 2010/4/30

Y1 - 2010/4/30

N2 - Approximately one-third of IVF cases in the UK are attributed to male factor infertility and in the majority of cases the origin of male infertility is unknown. The integrity of sperm DNA is important both for the success of assisted reproduction and the implications for the off-spring. One type of DNA damage that has not been investigated with respect to fertility outcomes is the adduct N7-methyldeoxyguanosine (N7-MedG), a biomarker for exposure to alkylating agents. A prospective cohort of couples attending for IVF had their N7-MedG levels in sperm measured using an immunoslot blot technique to examine whether sperm N7-MedG levels are associated with male factor infertility, semen quality measures or assisted reproduction outcomes. Sufficient DNA for analysis was obtained from 67/97 couples and N7-MedG was detected in 94% of sperm samples analysed. Men diagnosed with male factor infertility had significantly higher mean levels of N7-MedG in their sperm DNA (P=. 0.03). Logistic regression analysis showed that N7-MedG levels were significantly negatively associated with the proportion of oocytes successfully fertilised irrespective of the method of fertilisation used (IVF or intra-cytoplasmic sperm injection; ICSI, P

AB - Approximately one-third of IVF cases in the UK are attributed to male factor infertility and in the majority of cases the origin of male infertility is unknown. The integrity of sperm DNA is important both for the success of assisted reproduction and the implications for the off-spring. One type of DNA damage that has not been investigated with respect to fertility outcomes is the adduct N7-methyldeoxyguanosine (N7-MedG), a biomarker for exposure to alkylating agents. A prospective cohort of couples attending for IVF had their N7-MedG levels in sperm measured using an immunoslot blot technique to examine whether sperm N7-MedG levels are associated with male factor infertility, semen quality measures or assisted reproduction outcomes. Sufficient DNA for analysis was obtained from 67/97 couples and N7-MedG was detected in 94% of sperm samples analysed. Men diagnosed with male factor infertility had significantly higher mean levels of N7-MedG in their sperm DNA (P=. 0.03). Logistic regression analysis showed that N7-MedG levels were significantly negatively associated with the proportion of oocytes successfully fertilised irrespective of the method of fertilisation used (IVF or intra-cytoplasmic sperm injection; ICSI, P

KW - Alkylation

KW - DNA adducts

KW - DNA damage

KW - IVF

KW - Male infertility

KW - N7-methyldeoxyguanosine

U2 - 10.1016/j.mrgentox.2010.02.019

DO - 10.1016/j.mrgentox.2010.02.019

M3 - Article

C2 - 20307685

SN - 1383-5718

VL - 698

SP - 18

EP - 23

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

IS - 1-2

ER -

Alkylation of sperm DNA is associated with male factor infertility and a reduction in the proportion of oocytes fertilised during assisted reproduction

Abstract

Keywords

Access to Document

Fingerprint

Cite this