Alpha sarcoglycan is required for FGF-dependent myogenic progenitor cell proliferation in vitro and in vivo

Marco Cassano, Arianna Dellavalle, Francesco Saverio Tedesco, Mattia Quattrocelli, Stefania Crippa, Flavio Ronzoni, Agnese Salvade, Emanuele Berardi, Yvan Torrente, Giulio Cossu, Maurilio Sampaolesi

    Research output: Contribution to journalArticlepeer-review


    Mice deficient in α-sarcoglycan (Sgca-null mice) develop progressive muscular dystrophy and serve as a model for human limb girdle muscular dystrophy type 2D. Sgca-null mice suffer a more severe myopathy than that of mdx mice, the model for Duchenne muscular dystrophy. This is the opposite of what is observed in humans and the reason for this is unknown. In an attempt to understand the cellular basis of this severe muscular dystrophy, we isolated clonal populations of myogenic progenitor cells (MPCs), the resident postnatal muscle progenitors of dystrophic and wild-type mice. MPCs from Sgca-null mice generated much smaller clones than MPCs from wild-type or mdx dystrophic mice. Impaired proliferation of Sgca-null myogenic precursors was confirmed by single fiber analysis and this difference correlated with Sgca expression during MPC proliferation. In the absence of dystrophin and associated proteins, which are only expressed after differentiation, SGCA complexes with and stabilizes FGFR1. Deficiency of Sgca leads to an absence of FGFR1 expression at the membrane and impaired MPC proliferation in response to bFGF. The low proliferation rate of Sgca-null MPCs was rescued by transduction with Sgca-expressing lentiviral vectors. When transplanted into dystrophic muscle, Sgca-null MPCs exhibited reduced engraftment. The reduced proliferative ability of Sgca-null MPCs explains, at least in part, the severity of this muscular dystrophy and also why wild-type donor progenitor cells engraft efficiently and consequently ameliorate disease. © 2011. Published by The Company of Biologists Ltd.
    Original languageEnglish
    Pages (from-to)4523-4533
    Number of pages10
    Issue number20
    Publication statusPublished - 15 Oct 2011


    • Cell therapy
    • Limb girdle muscular dystrophy
    • Mouse
    • Myogenic progenitor cells


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