Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

Shankar Mukherjee; Fnu Nagajyothi; Aparna Mukhopadhyay; Fabiana S. Machado; Thomas J. Belbin; Antonio Campos de Carvalho; Fangxia Guan; Chris Albanese; Linda A. Jelicks; Michael P. Lisanti; Joao S. Silva; David C. Spray; Louis M. Weiss; Herbert B. Tanowitz

doi:10.1016/j.ygeno.2008.01.008

Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

Shankar Mukherjee, Fnu Nagajyothi, Aparna Mukhopadhyay, Fabiana S. Machado, Thomas J. Belbin, Antonio Campos de Carvalho, Fangxia Guan, Chris Albanese, Linda A. Jelicks, Michael P. Lisanti, Joao S. Silva, David C. Spray, Louis M. Weiss, Herbert B. Tanowitz

Research output: Contribution to journal › Article › peer-review

Abstract

Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention. © 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	423-432
Number of pages	9
Journal	Genomics
Volume	91
Issue number	5
DOIs	https://doi.org/10.1016/j.ygeno.2008.01.008
Publication status	Published - May 2008

Keywords

Cardiomyopathy
Chagas disease
Fas
Gene array analysis
Infection/inflammation
Trypanosoma cruzi; Cyclins

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10.1016/j.ygeno.2008.01.008

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Mukherjee, S., Nagajyothi, F., Mukhopadhyay, A., Machado, F. S., Belbin, T. J., Campos de Carvalho, A., Guan, F., Albanese, C., Jelicks, L. A., Lisanti, M. P., Silva, J. S., Spray, D. C., Weiss, L. M., & Tanowitz, H. B. (2008). Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection. Genomics, 91(5), 423-432. https://doi.org/10.1016/j.ygeno.2008.01.008

@article{4dd64644e15d4e71ae947d1384cda5eb,

title = "Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection",

abstract = "Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

keywords = "Cardiomyopathy, Chagas disease, Fas, Gene array analysis, Infection/inflammation, Trypanosoma cruzi; Cyclins",

author = "Shankar Mukherjee and Fnu Nagajyothi and Aparna Mukhopadhyay and Machado, {Fabiana S.} and Belbin, {Thomas J.} and {Campos de Carvalho}, Antonio and Fangxia Guan and Chris Albanese and Jelicks, {Linda A.} and Lisanti, {Michael P.} and Silva, {Joao S.} and Spray, {David C.} and Weiss, {Louis M.} and Tanowitz, {Herbert B.}",

note = "AI-052739, NIAID NIH HHS, United StatesAI-068538, NIAID NIH HHS, United StatesAI022730, NIAID NIH HHS, United StatesD43 TW007129-05, FIC NIH HHS, United StatesHL-073732, NHLBI NIH HHS, United StatesR01 AI052739-05, NIAID NIH HHS, United StatesR01 HL073732-05A1, NHLBI NIH HHS, United StatesR21 AI068538-01A1, NIAID NIH HHS, United States",

year = "2008",

month = may,

doi = "10.1016/j.ygeno.2008.01.008",

language = "English",

volume = "91",

pages = "423--432",

journal = "Genomics",

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Mukherjee, S, Nagajyothi, F, Mukhopadhyay, A, Machado, FS, Belbin, TJ, Campos de Carvalho, A, Guan, F, Albanese, C, Jelicks, LA, Lisanti, MP, Silva, JS, Spray, DC, Weiss, LM & Tanowitz, HB 2008, 'Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection', Genomics, vol. 91, no. 5, pp. 423-432. https://doi.org/10.1016/j.ygeno.2008.01.008

TY - JOUR

T1 - Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

AU - Mukherjee, Shankar

AU - Nagajyothi, Fnu

AU - Mukhopadhyay, Aparna

AU - Machado, Fabiana S.

AU - Belbin, Thomas J.

AU - Campos de Carvalho, Antonio

AU - Guan, Fangxia

AU - Albanese, Chris

AU - Jelicks, Linda A.

AU - Lisanti, Michael P.

AU - Silva, Joao S.

AU - Spray, David C.

AU - Weiss, Louis M.

AU - Tanowitz, Herbert B.

N1 - AI-052739, NIAID NIH HHS, United StatesAI-068538, NIAID NIH HHS, United StatesAI022730, NIAID NIH HHS, United StatesD43 TW007129-05, FIC NIH HHS, United StatesHL-073732, NHLBI NIH HHS, United StatesR01 AI052739-05, NIAID NIH HHS, United StatesR01 HL073732-05A1, NHLBI NIH HHS, United StatesR21 AI068538-01A1, NIAID NIH HHS, United States

PY - 2008/5

Y1 - 2008/5

N2 - Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention. © 2008 Elsevier Inc. All rights reserved.

AB - Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention. © 2008 Elsevier Inc. All rights reserved.

KW - Cardiomyopathy

KW - Chagas disease

KW - Fas

KW - Gene array analysis

KW - Infection/inflammation

KW - Trypanosoma cruzi; Cyclins

U2 - 10.1016/j.ygeno.2008.01.008

DO - 10.1016/j.ygeno.2008.01.008

M3 - Article

C2 - 18343633

SN - 0888-7543

VL - 91

SP - 423

EP - 432

JO - Genomics

JF - Genomics

IS - 5

ER -

Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

Abstract

Keywords

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