Altered cardiac sarcoplasmic reticulum function of intact myocytes of rat ventricle during metabolic inhibition

Stephen O'Neill; C. L. Overend; D. A. Eisner; S. C. O'Neill

doi:10.1161/01.RES.88.2.181

Altered cardiac sarcoplasmic reticulum function of intact myocytes of rat ventricle during metabolic inhibition

Stephen O'Neill, C. L. Overend, D. A. Eisner, S. C. O'Neill

Research output: Contribution to journal › Article › peer-review

Abstract

Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca2+ and the perforated patch-clamp technique to measure SR Ca2+ content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca2+ (sparks and waves of Ca2+-induced Ca2+ release), increased SR Ca2+ content (79.4±5.7 to 115.2±6.6 μmol/L cell volume [mean±SEM; P

Original language	English
Pages (from-to)	181-187
Number of pages	6
Journal	Circulation research
Volume	88
Issue number	2
DOIs	https://doi.org/10.1161/01.RES.88.2.181
Publication status	Published - 2 Feb 2001

Keywords

Calcium
Cardiac
Metabolic inhibition
Sarcoplasmic reticulum

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10.1161/01.RES.88.2.181

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abstract = "Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca2+ and the perforated patch-clamp technique to measure SR Ca2+ content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca2+ (sparks and waves of Ca2+-induced Ca2+ release), increased SR Ca2+ content (79.4±5.7 to 115.2±6.6 μmol/L cell volume [mean±SEM; P",

keywords = "Calcium, Cardiac, Metabolic inhibition, Sarcoplasmic reticulum",

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AU - O'Neill, Stephen

AU - Overend, C. L.

AU - Eisner, D. A.

AU - O'Neill, S. C.

PY - 2001/2/2

Y1 - 2001/2/2

N2 - Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca2+ and the perforated patch-clamp technique to measure SR Ca2+ content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca2+ (sparks and waves of Ca2+-induced Ca2+ release), increased SR Ca2+ content (79.4±5.7 to 115.2±6.6 μmol/L cell volume [mean±SEM; P

AB - Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca2+ and the perforated patch-clamp technique to measure SR Ca2+ content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca2+ (sparks and waves of Ca2+-induced Ca2+ release), increased SR Ca2+ content (79.4±5.7 to 115.2±6.6 μmol/L cell volume [mean±SEM; P

KW - Calcium

KW - Cardiac

KW - Metabolic inhibition

KW - Sarcoplasmic reticulum

U2 - 10.1161/01.RES.88.2.181

DO - 10.1161/01.RES.88.2.181

M3 - Article

SN - 1524-4571

VL - 88

SP - 181

EP - 187

JO - Circulation research

JF - Circulation research

IS - 2

ER -

Altered cardiac sarcoplasmic reticulum function of intact myocytes of rat ventricle during metabolic inhibition

Abstract

Keywords

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