Aminoglycoside antibiotics induce pH-sensitive activation of the calcium-sensing receptor

Stuart J. McLarnon; Darren Holden; Donald T. Ward; Malcolm N. Jones; Austin C. Elliott; Daniela Riccardi

doi:10.1016/S0006-291X(02)02133-2

Aminoglycoside antibiotics induce pH-sensitive activation of the calcium-sensing receptor

Stuart J. McLarnon, Darren Holden, Donald T. Ward, Malcolm N. Jones, Austin C. Elliott, Daniela Riccardi

Research output: Contribution to journal › Article › peer-review

Abstract

The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca2+ concentration ([Ca2+]i) using CaR-transfected human embryonic kidney (HEK)-293 cells. Gentamicin, tobramycin, and neomycin evoked dose- dependent increases in [Ca2+]i with EC50 values of 258, 177, and 43 μM, respectively, in CaR-transfected, but not in non-transfected cells. Kanamycin was ineffective at doses

Original language	English
Pages (from-to)	71-77
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	297
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(02)02133-2
Publication status	Published - 2002

Keywords

Calcium receptor
Fura-2 AM
Intracellular calcium
Intracellular pH

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10.1016/S0006-291X(02)02133-2

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title = "Aminoglycoside antibiotics induce pH-sensitive activation of the calcium-sensing receptor",

abstract = "The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca2+ concentration ([Ca2+]i) using CaR-transfected human embryonic kidney (HEK)-293 cells. Gentamicin, tobramycin, and neomycin evoked dose- dependent increases in [Ca2+]i with EC50 values of 258, 177, and 43 μM, respectively, in CaR-transfected, but not in non-transfected cells. Kanamycin was ineffective at doses",

keywords = "Calcium receptor, Fura-2 AM, Intracellular calcium, Intracellular pH",

author = "McLarnon, {Stuart J.} and Darren Holden and Ward, {Donald T.} and Jones, {Malcolm N.} and Elliott, {Austin C.} and Daniela Riccardi",

year = "2002",

doi = "10.1016/S0006-291X(02)02133-2",

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volume = "297",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Aminoglycoside antibiotics induce pH-sensitive activation of the calcium-sensing receptor

AU - McLarnon, Stuart J.

AU - Holden, Darren

AU - Ward, Donald T.

AU - Jones, Malcolm N.

AU - Elliott, Austin C.

AU - Riccardi, Daniela

PY - 2002

Y1 - 2002

N2 - The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca2+ concentration ([Ca2+]i) using CaR-transfected human embryonic kidney (HEK)-293 cells. Gentamicin, tobramycin, and neomycin evoked dose- dependent increases in [Ca2+]i with EC50 values of 258, 177, and 43 μM, respectively, in CaR-transfected, but not in non-transfected cells. Kanamycin was ineffective at doses

AB - The aminoglycoside antibiotic (AGA) neomycin is a known agonist of the extracellular calcium-sensing receptor (CaR). To test whether other AGA drugs stimulate the CaR, we studied the relative effects of four AGAs on intracellular Ca2+ concentration ([Ca2+]i) using CaR-transfected human embryonic kidney (HEK)-293 cells. Gentamicin, tobramycin, and neomycin evoked dose- dependent increases in [Ca2+]i with EC50 values of 258, 177, and 43 μM, respectively, in CaR-transfected, but not in non-transfected cells. Kanamycin was ineffective at doses

KW - Calcium receptor

KW - Fura-2 AM

KW - Intracellular calcium

KW - Intracellular pH

U2 - 10.1016/S0006-291X(02)02133-2

DO - 10.1016/S0006-291X(02)02133-2

M3 - Article

SN - 1090-2104

VL - 297

SP - 71

EP - 77

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Aminoglycoside antibiotics induce pH-sensitive activation of the calcium-sensing receptor

Abstract

Keywords

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