AMT (3-(2-aminopropyl)indole) and 5-IT (5-(2-aminopropyl)indole): An analytical challenge and implications for forensic analysis

Simon P. Elliott, Simon D. Brandt, Sally Freeman, Roland P. Archer

    Research output: Contribution to journalArticlepeer-review


    5-(2-Aminopropyl)indole (5-IT) and 3-(2-aminopropyl)indole (α-methyltryptamine, AMT) are isomeric substances and their differentiation can be a challenge under routine analytical conditions, especially when reference material is unavailable. 5-IT represents a very recent addition to the battery of new psychoactive substances that are commercially available from online retailers. This report illustrates how subtle differences observed under mass spectral and UV conditions can help to facilitate the differentiation between the two isomers. Analyses included 1H and 13C NMR, GC-EI/CI ion trap MS, applications of several U/HPLC-DAD and HPLC-MS methods. Investigations currently underway also highlight the confirmation that AMT was detected in a number of fatal intoxications. These findings also demonstrate that there is a potential risk of misidentification when dealing with both substances. © 2012 John Wiley & Sons, Ltd..
    Original languageEnglish
    Pages (from-to)196-202
    Number of pages6
    JournalDrug Testing and Analysis
    Issue number3
    Publication statusPublished - Mar 2013


    • 3-IT
    • 5-IT
    • AMT
    • Clinical
    • Forensic
    • Internet
    • Isomers
    • Legal highs
    • Tryptamines


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