An early evaluation of the HISTO SPOT® AB ID Class I & II test in cardiothoracic transplant patients

James Cashin, Patrick Flynn, Judith Worthington, Marcus Lowe, Andrew Canterbury, Kristin Launhardt, Ian Crosby, Stephen Sheldon, Rajamiyer Venkateswaran, Kay Poulton

Research output: Contribution to journalArticlepeer-review


The HISTO SPOT® AB ID assay (BAG Diagnostics GmbH) is a novel single antigen HLA Class I & II antibody definition test used with the MR.SPOT® processor. We compared this assay with Luminex® -based assays to assess its potential application in defining unacceptable antigens for transplantation in patients awaiting transplants with cardiothoracic organs. A cohort of 40 sensitized cardiothoracic patients were identified, and one sample was selected from each patient. The required screening was based on the patients' antibody profiles (Class I, n = 17, Class II, n = 11, Class I & II, n = 12). Samples were screened with LABScreen™ Single Antigen (SAg), LIFECODES® LSA™, HISTO SPOT® AB ID, and an acid modified LABScreen™ SAg test for detecting antibodies against denatured HLA. Results indicated that HISTO SPOT® AB ID had reduced sensitivity (68% for Class I; 69% for Class II). When compared to LABScreen™ and LIFECODES® , HISTO SPOT® AB ID failed to detect Luminex® -defined antibodies with median fluorescence intensity (MFI) ranging from 1114 to 24,489. The HISTO SPOT® AB ID panel used in the study had reduced antigen representation compared with Luminex® -based assays which further compromised its capacity for antibody detection and definition. Further work is needed to evaluate the clinical relevance of these differences between the performance of HISTO SPOT® and Luminex® -based methods.

Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalInternational Journal of Immunogenetics
Issue number5
Publication statusPublished - 1 Oct 2022


  • CTAG
  • Cardiothoracic
  • HLA antibody screening
  • LAB Screen
  • Luminex
  • Mr. Spot
  • Transplantation


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