TY - JOUR
T1 - An elisa method for the rapid and simple determination of aflatoxin in human serum
AU - Wilkinson, Andrew P.
AU - Morgan, Michael R.A.
AU - Denning, David W.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - A rapid, simple method for the determination of aflatoxin in human serum using anti-aflatoxin antisera in an indirect, double antibody microtitration plate enzyme-linked immunosorbent assay (ELISA) is described. Direct assay of serum necessitated the availability of a large sample volume, due to the presence of ‘non-specific’ interference. Addition of methanol to sera removed the interference enabling the analysis to be performed on small volumes (0-5 ml) of serum. The sensitivity of the assay is 20 pgml-1. Total assay time, including sample preparation, is 4-5h. No aflatoxin was found in any of the UK samples analysed.
AB - A rapid, simple method for the determination of aflatoxin in human serum using anti-aflatoxin antisera in an indirect, double antibody microtitration plate enzyme-linked immunosorbent assay (ELISA) is described. Direct assay of serum necessitated the availability of a large sample volume, due to the presence of ‘non-specific’ interference. Addition of methanol to sera removed the interference enabling the analysis to be performed on small volumes (0-5 ml) of serum. The sensitivity of the assay is 20 pgml-1. Total assay time, including sample preparation, is 4-5h. No aflatoxin was found in any of the UK samples analysed.
KW - Aflatoxin
KW - ELISA
KW - Human serum
UR - http://www.scopus.com/inward/record.url?scp=0023809329&partnerID=8YFLogxK
U2 - 10.1080/02652038809373725
DO - 10.1080/02652038809373725
M3 - Article
C2 - 3192012
AN - SCOPUS:0023809329
SN - 0265-203X
VL - 5
SP - 609
EP - 619
JO - Food Additives and Contaminants
JF - Food Additives and Contaminants
IS - 4
ER -