An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P

Michael Blatzer; Fabio Gsaller; Beate Abt; Markus Schrettl; Thomas Specht; Hubertus Haas

doi:10.1016/j.jbiotec.2013.11.003

An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P

Michael Blatzer, Fabio Gsaller, Beate Abt, Markus Schrettl, Thomas Specht, Hubertus Haas

Research output: Contribution to journal › Article › peer-review

Abstract

Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1P, is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1P-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1P promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus. © 2013 Elsevier B.V.

Original language	English
Pages (from-to)	82-86
Number of pages	4
Journal	Journal of biotechnology
Volume	169
Issue number	1
DOIs	https://doi.org/10.1016/j.jbiotec.2013.11.003
Publication status	Published - 10 Jan 2014

Keywords

Cephalosporin
Inducible promoter
Selection marker-recycling
Xylan/xylose-dependent gene expression
Xylanase

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10.1016/j.jbiotec.2013.11.003

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title = "An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P",

abstract = "Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1P, is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1P-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1P promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus. {\textcopyright} 2013 Elsevier B.V.",

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T1 - An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P

AU - Blatzer, Michael

AU - Gsaller, Fabio

AU - Abt, Beate

AU - Schrettl, Markus

AU - Specht, Thomas

AU - Haas, Hubertus

PY - 2014/1/10

Y1 - 2014/1/10

N2 - Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1P, is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1P-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1P promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus. © 2013 Elsevier B.V.

AB - Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1P, is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1P-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1P promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus. © 2013 Elsevier B.V.

KW - Cephalosporin

KW - Inducible promoter

KW - Selection marker-recycling

KW - Xylan/xylose-dependent gene expression

KW - Xylanase

U2 - 10.1016/j.jbiotec.2013.11.003

DO - 10.1016/j.jbiotec.2013.11.003

M3 - Article

C2 - 24246269

VL - 169

SP - 82

EP - 86

JO - Journal of biotechnology

JF - Journal of biotechnology

SN - 0168-1656

IS - 1

ER -

An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P

Abstract

Keywords

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