An endogenous promoter for conditional gene expression in Acremonium chrysogenum: The xylan and xylose inducible promoter xyl1P

Michael Blatzer, Fabio Gsaller, Beate Abt, Markus Schrettl, Thomas Specht, Hubertus Haas

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Acremonium chrysogenum is the natural producer of the beta-lactam antibiotic cephalosporin C and therefore of significant biotechnological importance. Here we identified and characterized the xylanase-encoding xyl1 gene and demonstrate that its promoter, xyl1P, is suitable for conditional expression of heterologous genes in A. chrysogenum. This was shown by xylose and xylan-inducible xyl1P-driven expression of genes encoding green fluorescence protein and phleomycin resistance. Moreover, we demonstrate the potential of the xyl1P promoter for selection marker recycling. Taken together, these finding will help to overcome the limitation in genetic tools in this important filamentous fungus. © 2013 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)82-86
    Number of pages4
    JournalJournal of biotechnology
    Volume169
    Issue number1
    DOIs
    Publication statusPublished - 10 Jan 2014

    Keywords

    • Cephalosporin
    • Inducible promoter
    • Selection marker-recycling
    • Xylan/xylose-dependent gene expression
    • Xylanase

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