An immune dot-blot technique for the diagnosis of ocular adenovirus infection

R Killough; P E Klapper; A S Bailey; I R Sharp; A Tullo; S J Richmond

doi:10.1016/0166-0934(90)90020-g

An immune dot-blot technique for the diagnosis of ocular adenovirus infection

R Killough, P E Klapper, A S Bailey, I R Sharp, A Tullo, S J Richmond

Booth Hall Children's Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

The development of a monoclonal antibody based radio-immune dot-blot technique (IDBT) for the rapid detection of adenovirus is described. 718 conjunctival swabs from patients with acute keratoconjunctivitis were examined by conventional cell culture isolation techniques and IDBT. IDBT identified adenovirus in 64 of 75 culture positive samples and also in a further 34 culture negative samples [Sensitivity (IDBT versus culture) 85.3%; Specificity 92.2%]. IDBT is considered to be a simple, clinically relevant, technique for the rapid identification of adenovirus infection of the eye.

Original language	English
Pages (from-to)	197-203
Number of pages	7
Journal	Journal of Virological Methods
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/0166-0934(90)90020-g
Publication status	Published - 1 Nov 1990

Keywords

Adenovirus Infections, Human/diagnosis
Adenoviruses, Human/isolation & purification
Animals
Antibodies, Monoclonal
Cell Line
Conjunctiva/microbiology
Eye Infections, Viral/diagnosis
Humans
Immunoblotting/methods
Radioimmunoassay/methods

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10.1016/0166-0934(90)90020-g

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title = "An immune dot-blot technique for the diagnosis of ocular adenovirus infection",

abstract = "The development of a monoclonal antibody based radio-immune dot-blot technique (IDBT) for the rapid detection of adenovirus is described. 718 conjunctival swabs from patients with acute keratoconjunctivitis were examined by conventional cell culture isolation techniques and IDBT. IDBT identified adenovirus in 64 of 75 culture positive samples and also in a further 34 culture negative samples [Sensitivity (IDBT versus culture) 85.3%; Specificity 92.2%]. IDBT is considered to be a simple, clinically relevant, technique for the rapid identification of adenovirus infection of the eye.",

keywords = "Adenovirus Infections, Human/diagnosis, Adenoviruses, Human/isolation & purification, Animals, Antibodies, Monoclonal, Cell Line, Conjunctiva/microbiology, Eye Infections, Viral/diagnosis, Humans, Immunoblotting/methods, Radioimmunoassay/methods",

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doi = "10.1016/0166-0934(90)90020-g",

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T1 - An immune dot-blot technique for the diagnosis of ocular adenovirus infection

AU - Killough, R

AU - Klapper, P E

AU - Bailey, A S

AU - Sharp, I R

AU - Tullo, A

AU - Richmond, S J

PY - 1990/11/1

Y1 - 1990/11/1

N2 - The development of a monoclonal antibody based radio-immune dot-blot technique (IDBT) for the rapid detection of adenovirus is described. 718 conjunctival swabs from patients with acute keratoconjunctivitis were examined by conventional cell culture isolation techniques and IDBT. IDBT identified adenovirus in 64 of 75 culture positive samples and also in a further 34 culture negative samples [Sensitivity (IDBT versus culture) 85.3%; Specificity 92.2%]. IDBT is considered to be a simple, clinically relevant, technique for the rapid identification of adenovirus infection of the eye.

AB - The development of a monoclonal antibody based radio-immune dot-blot technique (IDBT) for the rapid detection of adenovirus is described. 718 conjunctival swabs from patients with acute keratoconjunctivitis were examined by conventional cell culture isolation techniques and IDBT. IDBT identified adenovirus in 64 of 75 culture positive samples and also in a further 34 culture negative samples [Sensitivity (IDBT versus culture) 85.3%; Specificity 92.2%]. IDBT is considered to be a simple, clinically relevant, technique for the rapid identification of adenovirus infection of the eye.

KW - Adenovirus Infections, Human/diagnosis

KW - Adenoviruses, Human/isolation & purification

KW - Animals

KW - Antibodies, Monoclonal

KW - Cell Line

KW - Conjunctiva/microbiology

KW - Eye Infections, Viral/diagnosis

KW - Humans

KW - Immunoblotting/methods

KW - Radioimmunoassay/methods

U2 - 10.1016/0166-0934(90)90020-g

DO - 10.1016/0166-0934(90)90020-g

M3 - Article

C2 - 2262534

SN - 0166-0934

VL - 30

SP - 197

EP - 203

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

An immune dot-blot technique for the diagnosis of ocular adenovirus infection

Abstract

Keywords

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