An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants

Bridget V. Hogg; Joanna Kacprzyk; Elizabeth M. Molony; Conor O'Reilly; Thomas F. Gallagher; Patrick Gallois; Paul F. McCabe

doi:10.1186/1746-4811-7-45

An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants

Bridget V. Hogg, Joanna Kacprzyk, Elizabeth M. Molony, Conor O'Reilly, Thomas F. Gallagher, Patrick Gallois, Paul F. McCabe^*

^*Corresponding author for this work

Division of Molecular & Cellular Function (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation.

Original language	English
Article number	45
Journal	Plant Methods
Volume	7
Issue number	1
DOIs	https://doi.org/10.1186/1746-4811-7-45
Publication status	Published - 13 Dec 2011

Keywords

Apoptotic-like
Arabidopsis
Programmed cell death
Root hair

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10.1186/1746-4811-7-45

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abstract = "In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation.",

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AU - Molony, Elizabeth M.

AU - O'Reilly, Conor

AU - Gallagher, Thomas F.

AU - Gallois, Patrick

AU - McCabe, Paul F.

PY - 2011/12/13

Y1 - 2011/12/13

N2 - In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation.

AB - In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation.

KW - Apoptotic-like

KW - Arabidopsis

KW - Programmed cell death

KW - Root hair

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VL - 7

JO - Plant Methods

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ER -

An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants

Abstract

Keywords

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