An optical biosensor employing tiron-immobilised polypyrrole films for estimating monophenolase activity in apple juice.

Sabari Dutta; Subhash Padhye; R. Narayanaswamy; K. C. Persaud

doi:10.1016/S0956-5663(01)00136-1

An optical biosensor employing tiron-immobilised polypyrrole films for estimating monophenolase activity in apple juice.

Sabari Dutta, Subhash Padhye, R. Narayanaswamy, K. C. Persaud

Research output: Contribution to journal › Article › peer-review

Abstract

A method is described for the incorporation of tiron as a substrate for tyrosinase enzyme into a polypyrrole film deposited on indium titanium oxide (ITO) glass. The presence of tiron in the polypyrrole film is verified by cyclic voltammetry (CV). The enzyme activity using the polypyrrole-tiron film is confirmed by the catalytic conversion of immobilised substrate to quinones by the enzyme. The use of both potentiometric and optical methods for the detection of the catalytic activity of the polypyrrole-tiron film and their potential use for the determination of monophenolase activity of apple polyphenol oxidase is described. This is the first report of this kind whereby tiron has been immobilised in a polypyrrole matrix for the enzyme activity determination.

Original language	English
Pages (from-to)	287-294
Number of pages	7
Journal	Biosensors & bioelectronics
Volume	16
Issue number	4-5
DOIs	https://doi.org/10.1016/S0956-5663(01)00136-1
Publication status	Published - Jun 2001

Keywords

Polypyrrole
Tiron
Tyrosinase (polyphenol oxidase)

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10.1016/S0956-5663(01)00136-1

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title = "An optical biosensor employing tiron-immobilised polypyrrole films for estimating monophenolase activity in apple juice.",

abstract = "A method is described for the incorporation of tiron as a substrate for tyrosinase enzyme into a polypyrrole film deposited on indium titanium oxide (ITO) glass. The presence of tiron in the polypyrrole film is verified by cyclic voltammetry (CV). The enzyme activity using the polypyrrole-tiron film is confirmed by the catalytic conversion of immobilised substrate to quinones by the enzyme. The use of both potentiometric and optical methods for the detection of the catalytic activity of the polypyrrole-tiron film and their potential use for the determination of monophenolase activity of apple polyphenol oxidase is described. This is the first report of this kind whereby tiron has been immobilised in a polypyrrole matrix for the enzyme activity determination.",

keywords = "Polypyrrole, Tiron, Tyrosinase (polyphenol oxidase)",

author = "Sabari Dutta and Subhash Padhye and R. Narayanaswamy and Persaud, {K. C.}",

note = "Times Cited: 7",

year = "2001",

month = jun,

doi = "10.1016/S0956-5663(01)00136-1",

language = "English",

volume = "16",

pages = "287--294",

journal = "Biosensors & bioelectronics",

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}

TY - JOUR

T1 - An optical biosensor employing tiron-immobilised polypyrrole films for estimating monophenolase activity in apple juice.

AU - Dutta, Sabari

AU - Padhye, Subhash

AU - Narayanaswamy, R.

AU - Persaud, K. C.

N1 - Times Cited: 7

PY - 2001/6

Y1 - 2001/6

N2 - A method is described for the incorporation of tiron as a substrate for tyrosinase enzyme into a polypyrrole film deposited on indium titanium oxide (ITO) glass. The presence of tiron in the polypyrrole film is verified by cyclic voltammetry (CV). The enzyme activity using the polypyrrole-tiron film is confirmed by the catalytic conversion of immobilised substrate to quinones by the enzyme. The use of both potentiometric and optical methods for the detection of the catalytic activity of the polypyrrole-tiron film and their potential use for the determination of monophenolase activity of apple polyphenol oxidase is described. This is the first report of this kind whereby tiron has been immobilised in a polypyrrole matrix for the enzyme activity determination.

AB - A method is described for the incorporation of tiron as a substrate for tyrosinase enzyme into a polypyrrole film deposited on indium titanium oxide (ITO) glass. The presence of tiron in the polypyrrole film is verified by cyclic voltammetry (CV). The enzyme activity using the polypyrrole-tiron film is confirmed by the catalytic conversion of immobilised substrate to quinones by the enzyme. The use of both potentiometric and optical methods for the detection of the catalytic activity of the polypyrrole-tiron film and their potential use for the determination of monophenolase activity of apple polyphenol oxidase is described. This is the first report of this kind whereby tiron has been immobilised in a polypyrrole matrix for the enzyme activity determination.

KW - Polypyrrole

KW - Tiron

KW - Tyrosinase (polyphenol oxidase)

U2 - 10.1016/S0956-5663(01)00136-1

DO - 10.1016/S0956-5663(01)00136-1

M3 - Article

C2 - 11390216

SN - 1873-4235

VL - 16

SP - 287

EP - 294

JO - Biosensors & bioelectronics

JF - Biosensors & bioelectronics

IS - 4-5

ER -

An optical biosensor employing tiron-immobilised polypyrrole films for estimating monophenolase activity in apple juice.

Abstract

Keywords

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