Analysis of calcium oscillations in pancreatic acinar cells

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    Abstract

    Since they were first reported in hepatocytes in 1986, agonist-evoked oscillations in intracellular free calcium ([Ca2+](i)) have been described in numerous cell types, including pancreatic exocrine and endocrine cells. Pancreatic acinar cells show both 'sinusoidal' [Ca2+](i) oscillations (when stimulated with muscarinic agonists) and so-called 'baseline [Ca2+](i) spiking' (when stimulated with cholecystokinin, bombesin or analogues of either peptide). These agonist-evoked [Ca2+](i) oscillations present novel problems to the investigator, particularly since they show considerable cell-to-cell variability. In this review, we discuss the various approaches available for analysing [Ca2+](i) oscillations, particularly with respect to assessing whether drugs or other experimental interventions alter the oscillation pattern.
    Original languageEnglish
    Pages (from-to)69-74
    Number of pages5
    JournalDigestion
    Volume58
    Issue number2
    Publication statusPublished - Jul 1997

    Keywords

    • Acinar cell
    • Calcium
    • Calcium oscillation
    • Cholecystokinin
    • Pancreas
    • Secretagogue

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