Abstract
Since they were first reported in hepatocytes in 1986, agonist-evoked oscillations in intracellular free calcium ([Ca2+](i)) have been described in numerous cell types, including pancreatic exocrine and endocrine cells. Pancreatic acinar cells show both 'sinusoidal' [Ca2+](i) oscillations (when stimulated with muscarinic agonists) and so-called 'baseline [Ca2+](i) spiking' (when stimulated with cholecystokinin, bombesin or analogues of either peptide). These agonist-evoked [Ca2+](i) oscillations present novel problems to the investigator, particularly since they show considerable cell-to-cell variability. In this review, we discuss the various approaches available for analysing [Ca2+](i) oscillations, particularly with respect to assessing whether drugs or other experimental interventions alter the oscillation pattern.
Original language | English |
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Pages (from-to) | 69-74 |
Number of pages | 5 |
Journal | Digestion |
Volume | 58 |
Issue number | 2 |
Publication status | Published - Jul 1997 |
Keywords
- Acinar cell
- Calcium
- Calcium oscillation
- Cholecystokinin
- Pancreas
- Secretagogue