Analysis of microscopy and reconstructive images for applications in medicine and biology

G. Johnston; D. R. Burton; F. Lilley; A. Doyle; M. F. Murphy; G. Madden; M. A. Gdeisat; C. J. Moore; T. Marchant; B. Matuszewski

doi:10.1109/ICIP.2011.6116313

Analysis of microscopy and reconstructive images for applications in medicine and biology

G. Johnston
, D. R. Burton
, F. Lilley
, A. Doyle
, M. F. Murphy
, G. Madden
, M. A. Gdeisat
, C. J. Moore
, T. Marchant
, B. Matuszewski

Research output: Chapter in Book/Conference proceeding › Conference contribution › peer-review

Abstract

An investigation into orientation preferences shown by actin fibres within ex-situ actin as imaged by Atomic Force Microscopy (AFM) is described. Actin is a primary cytoskeletal component and is believed to play a vital role in cell structure. Actin structure images measured by AFM were analysed using automated pre-processing steps. These steps were identical for the production of an initial binary image, which was then processed by both Hough transformation and thinning. Results obtained question the validity of using the Hough transform approach, as bias could not easily be eliminated, and hence the Hough transform method was deemed to be unsuitable for this application and instead the thinning technique was used to identify and locate actin orientation within the AFM images. The results show that polymerised ex-situ actin fibres appears to display a bimodal distribution of orientation, with a 90 degree separation, with a significant co-efficient of bimodality of 0.656. © 2011 IEEE.

Original language	English
Title of host publication	Proceedings - International Conference on Image Processing, ICIP\|Proc. Int. Conf. Image Process. ICIP
Pages	3069-3072
Number of pages	3
DOIs	https://doi.org/10.1109/ICIP.2011.6116313
Publication status	Published - 2011
Event	2011 18th IEEE International Conference on Image Processing, ICIP 2011 - Brussels Duration: 1 Jul 2011 → … http://dx.doi.org/10.1109/ICIP.2011.6116317

Conference

Conference	2011 18th IEEE International Conference on Image Processing, ICIP 2011
City	Brussels
Period	1/07/11 → …
Internet address	http://dx.doi.org/10.1109/ICIP.2011.6116317

Keywords

Atomic force microscopy
feature extraction

Access to Document

10.1109/ICIP.2011.6116313

Cite this

Johnston, G., Burton, D. R., Lilley, F., Doyle, A., Murphy, M. F., Madden, G., Gdeisat, M. A., Moore, C. J., Marchant, T., & Matuszewski, B. (2011). Analysis of microscopy and reconstructive images for applications in medicine and biology. In Proceedings - International Conference on Image Processing, ICIP|Proc. Int. Conf. Image Process. ICIP (pp. 3069-3072) https://doi.org/10.1109/ICIP.2011.6116313

@inproceedings{caebe4d1177c43edaa812226cf5dfdeb,

title = "Analysis of microscopy and reconstructive images for applications in medicine and biology",

abstract = "An investigation into orientation preferences shown by actin fibres within ex-situ actin as imaged by Atomic Force Microscopy (AFM) is described. Actin is a primary cytoskeletal component and is believed to play a vital role in cell structure. Actin structure images measured by AFM were analysed using automated pre-processing steps. These steps were identical for the production of an initial binary image, which was then processed by both Hough transformation and thinning. Results obtained question the validity of using the Hough transform approach, as bias could not easily be eliminated, and hence the Hough transform method was deemed to be unsuitable for this application and instead the thinning technique was used to identify and locate actin orientation within the AFM images. The results show that polymerised ex-situ actin fibres appears to display a bimodal distribution of orientation, with a 90 degree separation, with a significant co-efficient of bimodality of 0.656. {\textcopyright} 2011 IEEE.",

keywords = "Atomic force microscopy, feature extraction",

author = "G. Johnston and Burton, \{D. R.\} and F. Lilley and A. Doyle and Murphy, \{M. F.\} and G. Madden and Gdeisat, \{M. A.\} and Moore, \{C. J.\} and T. Marchant and B. Matuszewski",

year = "2011",

doi = "10.1109/ICIP.2011.6116313",

language = "English",

isbn = "9781457713033",

pages = "3069--3072",

booktitle = "Proceedings - International Conference on Image Processing, ICIP|Proc. Int. Conf. Image Process. ICIP",

note = "2011 18th IEEE International Conference on Image Processing, ICIP 2011 ; Conference date: 01-07-2011",

url = "http://dx.doi.org/10.1109/ICIP.2011.6116317",

}

Johnston, G, Burton, DR, Lilley, F, Doyle, A, Murphy, MF, Madden, G, Gdeisat, MA, Moore, CJ, Marchant, T & Matuszewski, B 2011, Analysis of microscopy and reconstructive images for applications in medicine and biology. in Proceedings - International Conference on Image Processing, ICIP|Proc. Int. Conf. Image Process. ICIP. pp. 3069-3072, 2011 18th IEEE International Conference on Image Processing, ICIP 2011, Brussels, 1/07/11. https://doi.org/10.1109/ICIP.2011.6116313

TY - GEN

T1 - Analysis of microscopy and reconstructive images for applications in medicine and biology

AU - Johnston, G.

AU - Burton, D. R.

AU - Lilley, F.

AU - Doyle, A.

AU - Murphy, M. F.

AU - Madden, G.

AU - Gdeisat, M. A.

AU - Moore, C. J.

AU - Marchant, T.

AU - Matuszewski, B.

PY - 2011

Y1 - 2011

N2 - An investigation into orientation preferences shown by actin fibres within ex-situ actin as imaged by Atomic Force Microscopy (AFM) is described. Actin is a primary cytoskeletal component and is believed to play a vital role in cell structure. Actin structure images measured by AFM were analysed using automated pre-processing steps. These steps were identical for the production of an initial binary image, which was then processed by both Hough transformation and thinning. Results obtained question the validity of using the Hough transform approach, as bias could not easily be eliminated, and hence the Hough transform method was deemed to be unsuitable for this application and instead the thinning technique was used to identify and locate actin orientation within the AFM images. The results show that polymerised ex-situ actin fibres appears to display a bimodal distribution of orientation, with a 90 degree separation, with a significant co-efficient of bimodality of 0.656. © 2011 IEEE.

AB - An investigation into orientation preferences shown by actin fibres within ex-situ actin as imaged by Atomic Force Microscopy (AFM) is described. Actin is a primary cytoskeletal component and is believed to play a vital role in cell structure. Actin structure images measured by AFM were analysed using automated pre-processing steps. These steps were identical for the production of an initial binary image, which was then processed by both Hough transformation and thinning. Results obtained question the validity of using the Hough transform approach, as bias could not easily be eliminated, and hence the Hough transform method was deemed to be unsuitable for this application and instead the thinning technique was used to identify and locate actin orientation within the AFM images. The results show that polymerised ex-situ actin fibres appears to display a bimodal distribution of orientation, with a 90 degree separation, with a significant co-efficient of bimodality of 0.656. © 2011 IEEE.

KW - Atomic force microscopy

KW - feature extraction

U2 - 10.1109/ICIP.2011.6116313

DO - 10.1109/ICIP.2011.6116313

M3 - Conference contribution

SN - 9781457713033

SP - 3069

EP - 3072

BT - Proceedings - International Conference on Image Processing, ICIP|Proc. Int. Conf. Image Process. ICIP

T2 - 2011 18th IEEE International Conference on Image Processing, ICIP 2011

Y2 - 1 July 2011

ER -

Analysis of microscopy and reconstructive images for applications in medicine and biology

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Conference

Keywords

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