Analysis of the peptide content of the locust vasopressin-like immunoreactive (VPLI) neurons

Richard A. Baines; Kevin S J Thompson; Richard C. Rayne; Jonathan P. Bacon

Analysis of the peptide content of the locust vasopressin-like immunoreactive (VPLI) neurons

Richard A. Baines, Kevin S J Thompson, Richard C. Rayne, Jonathan P. Bacon

Research output: Contribution to journal › Article › peer-review

Abstract

Isolated cell bodies of the locust vasopressin-like immunoreactive (VPLI) neurons, analyzed by HPLC separation and radioimmune assay, contain three arginine vasopressin-like peptides: a previously identified monomer (F1, Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2) and its antiparallel homodimer (F2), but also the previously unreported parallel homodimer (PDm). VPLI neuron activity significantly reduces the level of cAMP in the CNS. Of the three synthetic peptides, only the monomer (F1, 10-8 and 10-6M) is capable of inhibiting a forskolin-stimulated increase in cAMP in isolated neural membranes. The antiparallel (F2) and parallel dimers (PDm) of this peptide have no effect on this second messenger. © 1995.

Original language	English
Pages (from-to)	799-807
Number of pages	8
Journal	Peptides
Volume	16
Issue number	5
Publication status	Published - 1995

Keywords

cAMP
Inhibition
Insect
Locusta migratoria
Neuropeptide

Cite this

@article{4cc17023d7854123a17c84262c26a2a2,

title = "Analysis of the peptide content of the locust vasopressin-like immunoreactive (VPLI) neurons",

abstract = "Isolated cell bodies of the locust vasopressin-like immunoreactive (VPLI) neurons, analyzed by HPLC separation and radioimmune assay, contain three arginine vasopressin-like peptides: a previously identified monomer (F1, Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2) and its antiparallel homodimer (F2), but also the previously unreported parallel homodimer (PDm). VPLI neuron activity significantly reduces the level of cAMP in the CNS. Of the three synthetic peptides, only the monomer (F1, 10-8 and 10-6M) is capable of inhibiting a forskolin-stimulated increase in cAMP in isolated neural membranes. The antiparallel (F2) and parallel dimers (PDm) of this peptide have no effect on this second messenger. {\textcopyright} 1995.",

keywords = "cAMP, Inhibition, Insect, Locusta migratoria, Neuropeptide",

author = "Baines, \{Richard A.\} and Thompson, \{Kevin S J\} and Rayne, \{Richard C.\} and Bacon, \{Jonathan P.\}",

year = "1995",

language = "English",

volume = "16",

pages = "799--807",

journal = "Peptides",

issn = "1873-5169",

publisher = "Elsevier BV",

number = "5",

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TY - JOUR

T1 - Analysis of the peptide content of the locust vasopressin-like immunoreactive (VPLI) neurons

AU - Baines, Richard A.

AU - Thompson, Kevin S J

AU - Rayne, Richard C.

AU - Bacon, Jonathan P.

PY - 1995

Y1 - 1995

N2 - Isolated cell bodies of the locust vasopressin-like immunoreactive (VPLI) neurons, analyzed by HPLC separation and radioimmune assay, contain three arginine vasopressin-like peptides: a previously identified monomer (F1, Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2) and its antiparallel homodimer (F2), but also the previously unreported parallel homodimer (PDm). VPLI neuron activity significantly reduces the level of cAMP in the CNS. Of the three synthetic peptides, only the monomer (F1, 10-8 and 10-6M) is capable of inhibiting a forskolin-stimulated increase in cAMP in isolated neural membranes. The antiparallel (F2) and parallel dimers (PDm) of this peptide have no effect on this second messenger. © 1995.

AB - Isolated cell bodies of the locust vasopressin-like immunoreactive (VPLI) neurons, analyzed by HPLC separation and radioimmune assay, contain three arginine vasopressin-like peptides: a previously identified monomer (F1, Cys-Leu-Ile-Thr-Asn-Cys-Pro-Arg-Gly-NH2) and its antiparallel homodimer (F2), but also the previously unreported parallel homodimer (PDm). VPLI neuron activity significantly reduces the level of cAMP in the CNS. Of the three synthetic peptides, only the monomer (F1, 10-8 and 10-6M) is capable of inhibiting a forskolin-stimulated increase in cAMP in isolated neural membranes. The antiparallel (F2) and parallel dimers (PDm) of this peptide have no effect on this second messenger. © 1995.

KW - cAMP

KW - Inhibition

KW - Insect

KW - Locusta migratoria

KW - Neuropeptide

UR - https://www.scopus.com/pages/publications/0029150089

M3 - Article

C2 - 7479318

SN - 1873-5169

VL - 16

SP - 799

EP - 807

JO - Peptides

JF - Peptides

IS - 5

ER -

Analysis of the peptide content of the locust vasopressin-like immunoreactive (VPLI) neurons

Abstract

Keywords

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