Analysis of the role of GADD153 in the control of apoptosis in NS0 myeloma cells

Idsada Lengwehasatit, Alan J. Dickson

    Research output: Contribution to journalArticlepeer-review


    Apoptosis can limit the maximum production of recombinant protein expression from cultured mammalian cells. This article focuses on the links between nutrient deprivation, ER perturbation, the regulation of (growth arrest and DNA damage inducible gene 153) GADD153 expression and apoptosis. During batch culture, decreases in glucose and glutamine correlated with an increase in apoptotic cells. This event was paralleled by a simultaneous increase in GADD153 expression. The expression of GADD153 in batch culture was suppressed by the addition of nutrients and with fed-batch culture the onset of apoptosis was delayed but not completely prevented. In defined stress conditions, glucose deprivation had the greatest effect on cell death when compared to glutamine deprivation or the addition of tunicamycin (an inhibitor of glycosylation), added to generate endoplasmic reticulum stress. However, the contribution of apoptosis to overall cell death (as judged by morphology) was smaller in conditions of glucose deprivation than in glutamine deprivation or tunicamycin treatment. Transient activation of GADD153 expression was found to occur in response to all stresses and occurred prior to detection of the onset of cell death. These results imply that GADD153 expression is either a trigger for apoptosis or offers a valid indicator of the likelihood of cell death arising from stresses of relevance to the bioreactor environment. © 2002 Wiley Periodicals, Inc.
    Original languageEnglish
    Pages (from-to)719-730
    Number of pages11
    JournalBiotechnology and Bioengineering
    Issue number7
    Publication statusPublished - 30 Dec 2002


    • Apoptosis
    • GADD153
    • NS0 myeloma cells
    • Nutrient deprivation
    • Stress


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