Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

Richard Bishop; Trushar Shah; Roger Pelle; David Hoyle; Terry Pearson; Lee Haines; Andrew Brass; Helen Hulme; Simon P. Graham; Evans L N Taracha; Simon Kanga; Charles Lu; Brian Hass; Jennifer Wortman; Owen White; Malcolm J. Gardner; Vishvanath Nene; Etienne P. de Villiers

doi:10.1093/nar/gki818

Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

Richard Bishop, Trushar Shah, Roger Pelle, David Hoyle, Terry Pearson, Lee Haines, Andrew Brass, Helen Hulme, Simon P. Graham, Evans L N Taracha, Simon Kanga, Charles Lu, Brian Hass, Jennifer Wortman, Owen White, Malcolm J. Gardner, Vishvanath Nene, Etienne P. de Villiers

Research output: Contribution to journal › Article › peer-review

Abstract

Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4-52 256 transcripts per million (t.p.m.). Rare transcripts (

Original language	English
Pages (from-to)	5503-5511
Number of pages	8
Journal	Nucleic acids research.
Volume	33
Issue number	17
DOIs	https://doi.org/10.1093/nar/gki818
Publication status	Published - 2005

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Bishop, R., Shah, T., Pelle, R., Hoyle, D., Pearson, T., Haines, L., Brass, A., Hulme, H., Graham, S. P., Taracha, E. L. N., Kanga, S., Lu, C., Hass, B., Wortman, J., White, O., Gardner, M. J., Nene, V., & de Villiers, E. P. (2005). Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage. Nucleic acids research., 33(17), 5503-5511. https://doi.org/10.1093/nar/gki818

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title = "Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage",

abstract = "Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4-52 256 transcripts per million (t.p.m.). Rare transcripts (",

author = "Richard Bishop and Trushar Shah and Roger Pelle and David Hoyle and Terry Pearson and Lee Haines and Andrew Brass and Helen Hulme and Graham, {Simon P.} and Taracha, {Evans L N} and Simon Kanga and Charles Lu and Brian Hass and Jennifer Wortman and Owen White and Gardner, {Malcolm J.} and Vishvanath Nene and {de Villiers}, {Etienne P.}",

year = "2005",

doi = "10.1093/nar/gki818",

language = "English",

volume = "33",

pages = "5503--5511",

journal = "Nucleic acids research.",

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publisher = "Oxford University Press",

number = "17",

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Bishop, R, Shah, T, Pelle, R, Hoyle, D, Pearson, T, Haines, L, Brass, A, Hulme, H, Graham, SP, Taracha, ELN, Kanga, S, Lu, C, Hass, B, Wortman, J, White, O, Gardner, MJ, Nene, V & de Villiers, EP 2005, 'Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage', Nucleic acids research., vol. 33, no. 17, pp. 5503-5511. https://doi.org/10.1093/nar/gki818

TY - JOUR

T1 - Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

AU - Bishop, Richard

AU - Shah, Trushar

AU - Pelle, Roger

AU - Hoyle, David

AU - Pearson, Terry

AU - Haines, Lee

AU - Brass, Andrew

AU - Hulme, Helen

AU - Graham, Simon P.

AU - Taracha, Evans L N

AU - Kanga, Simon

AU - Lu, Charles

AU - Hass, Brian

AU - Wortman, Jennifer

AU - White, Owen

AU - Gardner, Malcolm J.

AU - Nene, Vishvanath

AU - de Villiers, Etienne P.

PY - 2005

Y1 - 2005

N2 - Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4-52 256 transcripts per million (t.p.m.). Rare transcripts (

AB - Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4-52 256 transcripts per million (t.p.m.). Rare transcripts (

U2 - 10.1093/nar/gki818

DO - 10.1093/nar/gki818

M3 - Article

SN - 1362-4962

VL - 33

SP - 5503

EP - 5511

JO - Nucleic acids research.

JF - Nucleic acids research.

IS - 17

ER -

Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage

Abstract

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