Azole resistance in Aspergillus fumigatus is on the rise. Non-target mediated mechanisms are a common cause of azole resistance in chronic pulmonary aspergillosis (CPA). Here we investigate resistance mechanisms using whole genome sequencing. Sixteen azole-resistant A. fumigatus isolates from CPA were sequenced to assess genome rearrangements. 7/16 CPA isolates showed genomic duplications compared to 0/18 invasive isolates. Duplication of regions including cyp51A increased gene expression. Our results suggest aneuploidy as an azole resistance mechanism in CPA.
|Journal||Antimicrobial Agents and Chemotherapy|
|Publication status||Accepted/In press - 9 Feb 2023|