Angiogenesis is induced and wound size is reduced by electrical stimulation in an acute wound healing model in human skin.

S Ud-Din, A Sebastian, P Giddings, J Colthurst, S Whiteside, J Morris, R Nuccitelli, C Pullar, M Baguneid, A Bayat

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Angiogenesis is critical for wound healing. Insufficient angiogenesis can result in impaired wound healing and chronic wound formation. Electrical stimulation (ES) has been shown to enhance angiogenesis. We previously showed that ES enhanced angiogenesis in acute wounds at one time point (day 14). The aim of this study was to further evaluate the role of ES in affecting angiogenesis during the acute phase of cutaneous wound healing over multiple time points. We compared the angiogenic response to wounding in 40 healthy volunteers (divided into two groups and randomised), treated with ES (post-ES) and compared them to secondary intention wound healing (control). Biopsy time points monitored were days 0, 3, 7, 10, 14. Objective non-invasive measures and H&E analysis were performed in addition to immunohistochemistry (IHC) and Western blotting (WB). Wound volume was significantly reduced on D7, 10 and 14 post-ES (p = 0.003, p = 0.002, p0.05) on day 14 in VEGF-A expression post-ES compared to controls. Furthermore, organisation of granulation tissue was improved on day 14 post-ES. This randomised controlled trial has shown that ES enhanced wound healing by reduced wound dimensions and increased VEGF-A and PLGF expression in acute cutaneous wounds, which further substantiates the role of ES in up-regulating angiogenesis as observed over multiple time points. This therapeutic approach may have potential application for clinical management of delayed and chronic wounds.
    Original languageEnglish
    Pages (from-to)1-22
    Number of pages21
    JournalP L o S One
    Volume10
    Issue number4
    DOIs
    Publication statusPublished - 30 Apr 2015

    Keywords

    • wound healing
    • electrical stimulation
    • angiogenesis
    • accelerated rate of repair

    Fingerprint

    Dive into the research topics of 'Angiogenesis is induced and wound size is reduced by electrical stimulation in an acute wound healing model in human skin.'. Together they form a unique fingerprint.

    Cite this