Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches

Pinar Pir; Kutlu Ö Ülgen; Andrew Hayes; Ilsen Önsan; Betül Kirdar; Stephen G. Oliver

doi:10.1002/yea.1375

Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches

Pinar Pir, Kutlu Ö Ülgen, Andrew Hayes, Ilsen Önsan, Betül Kirdar, Stephen G. Oliver

Research output: Contribution to journal › Article › peer-review

Abstract

Changes in the expression of genes were used to elucidate the metabolic pathways and regulatory mechanisms that respond to environmental or genetic modifications. Results from previously published chemostat datasets were merged with novel data generated in the present study. ORFs displaying significant changes in expression that correlated with those of other ORFs were analysed using GO mapping tools and supplemented by literature information. The strategy developed was used to propose annotations for ORFs of unknown function. The following ORFs were assigned functions as a result of this study: YMR090w, YGL157w, YGR243w, YLR327c, YER121w, YFR017c, YGR067c, YKL187c, YGR236c (SPG1), YMR107w (SPG4), YMR206w, YER067w, YJL103c, YNL175C (NOP13) YJL200C, YDL070C (FMP16) and YGR173W. Copyright © 2006 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	553-571
Number of pages	18
Journal	Yeast
Volume	23
Issue number	7
DOIs	https://doi.org/10.1002/yea.1375
Publication status	Published - May 2006

Keywords

Correlation analysis
Functional annotation
GO mapping
Linear modelling
Saccharomyces cerevisiae
Transcriptome

Research Beacons, Institutes and Platforms

Manchester Institute of Biotechnology

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10.1002/yea.1375

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@article{a7e95649987f4c058871b96eb454262d,

title = "Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches",

abstract = "Changes in the expression of genes were used to elucidate the metabolic pathways and regulatory mechanisms that respond to environmental or genetic modifications. Results from previously published chemostat datasets were merged with novel data generated in the present study. ORFs displaying significant changes in expression that correlated with those of other ORFs were analysed using GO mapping tools and supplemented by literature information. The strategy developed was used to propose annotations for ORFs of unknown function. The following ORFs were assigned functions as a result of this study: YMR090w, YGL157w, YGR243w, YLR327c, YER121w, YFR017c, YGR067c, YKL187c, YGR236c (SPG1), YMR107w (SPG4), YMR206w, YER067w, YJL103c, YNL175C (NOP13) YJL200C, YDL070C (FMP16) and YGR173W. Copyright {\textcopyright} 2006 John Wiley & Sons, Ltd.",

keywords = "Correlation analysis, Functional annotation, GO mapping, Linear modelling, Saccharomyces cerevisiae, Transcriptome",

author = "Pinar Pir and {\"U}lgen, {Kutlu {\"O}} and Andrew Hayes and Ilsen {\"O}nsan and Bet{\"u}l Kirdar and Oliver, {Stephen G.}",

year = "2006",

month = may,

doi = "10.1002/yea.1375",

language = "English",

volume = "23",

pages = "553--571",

journal = "Yeast",

issn = "1097-0061",

publisher = "John Wiley & Sons Ltd",

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TY - JOUR

T1 - Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches

AU - Pir, Pinar

AU - Ülgen, Kutlu Ö

AU - Hayes, Andrew

AU - Önsan, Ilsen

AU - Kirdar, Betül

AU - Oliver, Stephen G.

PY - 2006/5

Y1 - 2006/5

N2 - Changes in the expression of genes were used to elucidate the metabolic pathways and regulatory mechanisms that respond to environmental or genetic modifications. Results from previously published chemostat datasets were merged with novel data generated in the present study. ORFs displaying significant changes in expression that correlated with those of other ORFs were analysed using GO mapping tools and supplemented by literature information. The strategy developed was used to propose annotations for ORFs of unknown function. The following ORFs were assigned functions as a result of this study: YMR090w, YGL157w, YGR243w, YLR327c, YER121w, YFR017c, YGR067c, YKL187c, YGR236c (SPG1), YMR107w (SPG4), YMR206w, YER067w, YJL103c, YNL175C (NOP13) YJL200C, YDL070C (FMP16) and YGR173W. Copyright © 2006 John Wiley & Sons, Ltd.

AB - Changes in the expression of genes were used to elucidate the metabolic pathways and regulatory mechanisms that respond to environmental or genetic modifications. Results from previously published chemostat datasets were merged with novel data generated in the present study. ORFs displaying significant changes in expression that correlated with those of other ORFs were analysed using GO mapping tools and supplemented by literature information. The strategy developed was used to propose annotations for ORFs of unknown function. The following ORFs were assigned functions as a result of this study: YMR090w, YGL157w, YGR243w, YLR327c, YER121w, YFR017c, YGR067c, YKL187c, YGR236c (SPG1), YMR107w (SPG4), YMR206w, YER067w, YJL103c, YNL175C (NOP13) YJL200C, YDL070C (FMP16) and YGR173W. Copyright © 2006 John Wiley & Sons, Ltd.

KW - Correlation analysis

KW - Functional annotation

KW - GO mapping

KW - Linear modelling

KW - Saccharomyces cerevisiae

KW - Transcriptome

U2 - 10.1002/yea.1375

DO - 10.1002/yea.1375

M3 - Article

C2 - 16710832

SN - 1097-0061

VL - 23

SP - 553

EP - 571

JO - Yeast

JF - Yeast

IS - 7

ER -

Annotation of unknown yeast ORFs by correlation analysis of microarray data and extensive literature searches

Abstract

Keywords

Research Beacons, Institutes and Platforms

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