Antibody development and use in chromogenic and fluorescent immunostaining.

Eamon Dubaissi; Niki Panagiotaki; Nancy Papalopulu; Peter D Vize

doi:10.1007/978-1-61779-992-1_23

Antibody development and use in chromogenic and fluorescent immunostaining.

Eamon Dubaissi, Niki Panagiotaki, Nancy Papalopulu, Peter D Vize

Research output: Contribution to journal › Article › peer-review

Abstract

Antibody-based detection of protein distribution patterns both in wholemount and on sections revolutionized Xenopus research and ushered in the visual-based era of Xenopus data presentation. The ability to view the distribution of a gene product throughout an embryo makes it possible to rapidly map normal expression profiles and profiles that have been altered by an experimental intervention. The main limiting element in Xenopus immunostaining techniques has always been the availability of antibodies that work well on fixed whole embryos, a problem that persists. However, new antibodies are constantly being generated and improvements in detection systems allow antibodies that were once below the limits of detection to be utilized in multichannel immunofluorescence using tyramide amplification.

Original language	English
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	917
DOIs	https://doi.org/10.1007/978-1-61779-992-1_23
Publication status	Published - 2012

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abstract = "Antibody-based detection of protein distribution patterns both in wholemount and on sections revolutionized Xenopus research and ushered in the visual-based era of Xenopus data presentation. The ability to view the distribution of a gene product throughout an embryo makes it possible to rapidly map normal expression profiles and profiles that have been altered by an experimental intervention. The main limiting element in Xenopus immunostaining techniques has always been the availability of antibodies that work well on fixed whole embryos, a problem that persists. However, new antibodies are constantly being generated and improvements in detection systems allow antibodies that were once below the limits of detection to be utilized in multichannel immunofluorescence using tyramide amplification.",

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AU - Panagiotaki, Niki

AU - Papalopulu, Nancy

AU - Vize, Peter D

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Antibody development and use in chromogenic and fluorescent immunostaining.

Abstract

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