Antigen and antibody attachment in ELISA for Aspergillus fumigatus IgG antibodies

M. D. Richardson, D. W. Warnock

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    Four commercial antigen extracts of Aspergillus fumigatus were evaluated for use in a rapid enzyme-linked immunosorbent assay (ELISA) for anti-A. fumigatus IgG. Initial binding of both somatic and culture filtrate preparations to a polyvinyl chloride solid phase was concentration dependent and increased with incubation time. Antigen binding to the solid phase was reproducible. Binding of A. fumigatus precipitin-positive serum to bound antigen was rapid. All four A. fumigatus antigens demonstrated similar dose-response curves when tested against pooled sera containing a high titre of A. fumigatus antibodies. Detectable activity in precipitin test-negative sera decreased rapidly with dilution. All the antigen preparations were found to be suitable for ELISA procedures and permit the rapid determination of IgG antibodies to A. fumigatus. © 1984.
    Original languageEnglish
    Pages (from-to)119-132
    Number of pages13
    JournalJournal of immunological methods
    Issue number1
    Publication statusPublished - 1984


    • aspergillosis
    • Aspergillus fumigatus antigens
    • ELISA methodology


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