Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces

Leigh Townsend; Richard L. Williams; Olachi Anuforom; Matthew R. Berwick; Fenella Halstead; Erik Hughes; Artemis Stamboulis; Beryl Oppenheim; Julie Gough; Liam Grover; Robert A H Scott; Mark Webber; Anna F A Peacock; Antonio Belli; Ann Logan; Felicity De Cogan

doi:10.1098/rsif.2016.0657

Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces

Leigh Townsend, Richard L. Williams, Olachi Anuforom, Matthew R. Berwick, Fenella Halstead, Erik Hughes, Artemis Stamboulis, Beryl Oppenheim, Julie Gough, Liam Grover, Robert A H Scott, Mark Webber, Anna F A Peacock, Antonio Belli, Ann Logan, Felicity De Cogan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material-tissue interface with an antimicrobial peptide (AMP) coating to allowcell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists ofAMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

Original language	English
Article number	20160657
Journal	Journal of the Royal Society Interface
Volume	14
Issue number	126
DOIs	https://doi.org/10.1098/rsif.2016.0657
Publication status	Published - 11 Jan 2017

Keywords

Antimicrobial
Device-tissue interface
Surface engineering

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10.1098/rsif.2016.0657

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Townsend, L., Williams, R. L., Anuforom, O., Berwick, M. R., Halstead, F., Hughes, E., Stamboulis, A., Oppenheim, B., Gough, J., Grover, L., Scott, R. A. H., Webber, M., Peacock, A. F. A., Belli, A., Logan, A., & De Cogan, F. (2017). Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces. Journal of the Royal Society Interface, 14(126), Article 20160657. https://doi.org/10.1098/rsif.2016.0657

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abstract = "The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material-tissue interface with an antimicrobial peptide (AMP) coating to allowcell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists ofAMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.",

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Townsend, L, Williams, RL, Anuforom, O, Berwick, MR, Halstead, F, Hughes, E, Stamboulis, A, Oppenheim, B, Gough, J, Grover, L, Scott, RAH, Webber, M, Peacock, AFA, Belli, A, Logan, A & De Cogan, F 2017, 'Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces', Journal of the Royal Society Interface, vol. 14, no. 126, 20160657. https://doi.org/10.1098/rsif.2016.0657

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T1 - Antimicrobial peptide coatings for hydroxyapatite

T2 - Electrostatic and covalent attachment of antimicrobial peptides to surfaces

AU - Townsend, Leigh

AU - Williams, Richard L.

AU - Anuforom, Olachi

AU - Berwick, Matthew R.

AU - Halstead, Fenella

AU - Hughes, Erik

AU - Stamboulis, Artemis

AU - Oppenheim, Beryl

AU - Gough, Julie

AU - Grover, Liam

AU - Scott, Robert A H

AU - Webber, Mark

AU - Peacock, Anna F A

AU - Belli, Antonio

AU - Logan, Ann

AU - De Cogan, Felicity

PY - 2017/1/11

Y1 - 2017/1/11

N2 - The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material-tissue interface with an antimicrobial peptide (AMP) coating to allowcell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists ofAMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

AB - The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material-tissue interface with an antimicrobial peptide (AMP) coating to allowcell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists ofAMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.

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KW - Surface engineering

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Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces

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Antimicrobial peptide coatings for hydroxyapatite: Electrostatic and covalent attachment of antimicrobial peptides to surfaces

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Supplementary material from "Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces"

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