Antioxidant activity and ACE-inhibitory of Class II hydrophobin from wild strain Trichoderma reesei

Mohammadreza Khalesi, Raheleh Jahanbani, David Riveros-Galan, Vahid Sheikh-Hassani, Mahmoud Sheikh-Zeinoddin, Mehdi Sahihi, James Winterburn, Guy Derdelinckx, Ali Akbar Moosavi-Movahedi

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    There are several possible uses of the Class II hydrophobin
    HFBII in clinical applications. To fully understand and exploit this
    potential however, the antioxidant activity and ACE-inhibitory potential
    of this protein need to be better understood and have not been previously
    reported. In this study, the Class II hydrophobin HFBII was produced by
    the cultivation of wild type Trichoderma reesei. The crude hydrophobin
    extract obtained from the fermentation process was purified using
    reversed-phase liquid chromatography and the identity of the purified
    HFBII verified by MALDI-TOF (molecular weight: 7.2 kDa). Subsequently the
    antioxidant activity of different concentrations of HFBII (0.01-0.40
    mg/mL) were determined. The results show that for HFBII concentrations of
    0.04 mg/mL and upwards the protein significantly reduced the presence of
    ABTS+ radicals in the medium, the IC50 value found to be 0.13 mg/mL.
    Computational modeling highlighted the role of the amino acid residues
    located in the conserved and exposed hydrophobic patch on the surface of
    the HFBII molecule and the interactions with the aromatic rings of ABTS.
    The ACE-inhibitory effect of HFBII was found to occur from 0.5 mg/mL and
    upwards, making the combination of HFBII with strong ACE-inhibitors
    attractive for use in the healthcare industry
    Original languageEnglish
    JournalInternational Journal of Biological Macromolecules
    Early online date19 May 2016
    Publication statusPublished - Oct 2016


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