Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells.

Christoph Ballestrem; Benjamin Geiger

doi:10.1385/1-59259-860-9:321

Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells.

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Abstract

This chapter describes the use of microscope-based fluorescence resonance energy transfer to follow dynamic interaction of molecules localized at focal adhesions. We first outline the significance of studying dynamic interactions in focal adhesions of living cells, and second provide an overview of the method itself. This is followed by a protocol for fluorescence resonance energy transfer measurements in live cells.

Original language	English
Pages (from-to)	321-334
Number of pages	13
Journal	Methods in Molecular Biology
Volume	294
DOIs	https://doi.org/10.1385/1-59259-860-9:321
Publication status	Published - 2005

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title = "Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells.",

abstract = "This chapter describes the use of microscope-based fluorescence resonance energy transfer to follow dynamic interaction of molecules localized at focal adhesions. We first outline the significance of studying dynamic interactions in focal adhesions of living cells, and second provide an overview of the method itself. This is followed by a protocol for fluorescence resonance energy transfer measurements in live cells.",

author = "Christoph Ballestrem and Benjamin Geiger",

year = "2005",

doi = "10.1385/1-59259-860-9:321",

language = "English",

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AU - Geiger, Benjamin

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AB - This chapter describes the use of microscope-based fluorescence resonance energy transfer to follow dynamic interaction of molecules localized at focal adhesions. We first outline the significance of studying dynamic interactions in focal adhesions of living cells, and second provide an overview of the method itself. This is followed by a protocol for fluorescence resonance energy transfer measurements in live cells.

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DO - 10.1385/1-59259-860-9:321

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SN - 1064-3745

VL - 294

SP - 321

EP - 334

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells.

Abstract

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