Aptamer evolution for array-based diagnostics

Mark Platt; William Rowe; David C. Wedge; Douglas B. Kell; Joshua Knowles; Philip J R Day

doi:10.1016/j.ab.2009.04.013

Aptamer evolution for array-based diagnostics

Mark Platt, William Rowe, David C. Wedge, Douglas B. Kell, Joshua Knowles, Philip J R Day

Research output: Contribution to journal › Article › peer-review

Abstract

Closed loop aptameric directed evolution, (CLADE) is a technique enabling simultaneous discovery, evolution, and optimization of aptamers. It was previously demonstrated using a fluorescent protein, and here we extend its applicability with the generation of surface-bound aptamers for targets containing no natural fluorescence. Starting from a random population, in four generations CLADE produced a new aptamer to thrombin with high specificity and affinity. The best aptameric sequence was void of the set of four guanine repeats typifying thrombin aptamers and, thus, highlights the benefits of evolution performed in an environment closely mimicking the final diagnostic application. © 2009 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	203-205
Number of pages	2
Journal	Analytical Biochemistry
Volume	390
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2009.04.013
Publication status	Published - 15 Jul 2009

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title = "Aptamer evolution for array-based diagnostics",

abstract = "Closed loop aptameric directed evolution, (CLADE) is a technique enabling simultaneous discovery, evolution, and optimization of aptamers. It was previously demonstrated using a fluorescent protein, and here we extend its applicability with the generation of surface-bound aptamers for targets containing no natural fluorescence. Starting from a random population, in four generations CLADE produced a new aptamer to thrombin with high specificity and affinity. The best aptameric sequence was void of the set of four guanine repeats typifying thrombin aptamers and, thus, highlights the benefits of evolution performed in an environment closely mimicking the final diagnostic application. {\textcopyright} 2009 Elsevier Inc. All rights reserved.",

author = "Mark Platt and William Rowe and Wedge, {David C.} and Kell, {Douglas B.} and Joshua Knowles and Day, {Philip J R}",

note = "This work was supported by the Manchester Center for Integrative Systems Biology and was sponsored by the Biotechnology and Biological Sciences Research Council (BBSRC, PBB/D000203/1).",

year = "2009",

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doi = "10.1016/j.ab.2009.04.013",

language = "English",

volume = "390",

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journal = "Analytical Biochemistry",

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T1 - Aptamer evolution for array-based diagnostics

AU - Platt, Mark

AU - Rowe, William

AU - Wedge, David C.

AU - Kell, Douglas B.

AU - Knowles, Joshua

AU - Day, Philip J R

N1 - This work was supported by the Manchester Center for Integrative Systems Biology and was sponsored by the Biotechnology and Biological Sciences Research Council (BBSRC, PBB/D000203/1).

PY - 2009/7/15

Y1 - 2009/7/15

N2 - Closed loop aptameric directed evolution, (CLADE) is a technique enabling simultaneous discovery, evolution, and optimization of aptamers. It was previously demonstrated using a fluorescent protein, and here we extend its applicability with the generation of surface-bound aptamers for targets containing no natural fluorescence. Starting from a random population, in four generations CLADE produced a new aptamer to thrombin with high specificity and affinity. The best aptameric sequence was void of the set of four guanine repeats typifying thrombin aptamers and, thus, highlights the benefits of evolution performed in an environment closely mimicking the final diagnostic application. © 2009 Elsevier Inc. All rights reserved.

AB - Closed loop aptameric directed evolution, (CLADE) is a technique enabling simultaneous discovery, evolution, and optimization of aptamers. It was previously demonstrated using a fluorescent protein, and here we extend its applicability with the generation of surface-bound aptamers for targets containing no natural fluorescence. Starting from a random population, in four generations CLADE produced a new aptamer to thrombin with high specificity and affinity. The best aptameric sequence was void of the set of four guanine repeats typifying thrombin aptamers and, thus, highlights the benefits of evolution performed in an environment closely mimicking the final diagnostic application. © 2009 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.ab.2009.04.013

DO - 10.1016/j.ab.2009.04.013

M3 - Article

SN - 0003-2697

VL - 390

SP - 203

EP - 205

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Aptamer evolution for array-based diagnostics

Abstract

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