Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM

Yuli Xu, Tamzin Gristwood, Benjamin Hodgson, Jonathan C. Trinidad, Sonja Verena Albers, Stephen D. Bella

Research output: Contribution to journalArticlepeer-review

Abstract

The regulated recruitment of Cdc45 and GINS is key to activating the eukaryotic MCM(2-7) replicative helicase. We demonstrate that the homohexameric archaeal MCM helicase associates with orthologs of GINS and Cdc45 in vivo and in vitro. Association of these factors with MCM robustly stimulates the MCM helicase activity. In contrast to the situation in eukaryotes, archaeal Cdc45 and GINS form an extremely stable complex before binding MCM. Further, the archaeal GINS•Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome.
Original languageEnglish
JournalPNAS
DOIs
Publication statusPublished - 7 Nov 2016

Research Beacons, Institutes and Platforms

  • Manchester Cancer Research Centre

Fingerprint

Dive into the research topics of 'Archaeal orthologs of Cdc45 and GINS form a stable complex that stimulates the helicase activity of MCM'. Together they form a unique fingerprint.

Cite this