Array-based diagnostic gene-expression score for atopy and asthma

Martin H. Brutsche; Ladina Joos; Ingrid E A Carlen Brutsche; Roland Bissinger; Michael Tamm; Adnan Custovic; Ashley Woodcock

doi:10.1067/mai.2002.121530

Array-based diagnostic gene-expression score for atopy and asthma

Martin H. Brutsche, Ladina Joos, Ingrid E A Carlen Brutsche, Roland Bissinger, Michael Tamm, Adnan Custovic, Ashley Woodcock

Research output: Contribution to journal › Article › peer-review

Abstract

Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.

Original language	English
Pages (from-to)	271-273
Number of pages	2
Journal	Journal of Allergy and Clinical Immunology
Volume	109
Issue number	2
DOIs	https://doi.org/10.1067/mai.2002.121530
Publication status	Published - 2002

Keywords

Asthma
Gene expression
Health-status indicators
Respiratory hypersensitivity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1067/mai.2002.121530

Cite this

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title = "Array-based diagnostic gene-expression score for atopy and asthma",

abstract = "Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.",

keywords = "Asthma, Gene expression, Health-status indicators, Respiratory hypersensitivity",

author = "Brutsche, {Martin H.} and Ladina Joos and {Carlen Brutsche}, {Ingrid E A} and Roland Bissinger and Michael Tamm and Adnan Custovic and Ashley Woodcock",

year = "2002",

doi = "10.1067/mai.2002.121530",

language = "English",

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pages = "271--273",

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T1 - Array-based diagnostic gene-expression score for atopy and asthma

AU - Brutsche, Martin H.

AU - Joos, Ladina

AU - Carlen Brutsche, Ingrid E A

AU - Bissinger, Roland

AU - Tamm, Michael

AU - Custovic, Adnan

AU - Woodcock, Ashley

PY - 2002

Y1 - 2002

N2 - Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.

AB - Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.

KW - Asthma

KW - Gene expression

KW - Health-status indicators

KW - Respiratory hypersensitivity

U2 - 10.1067/mai.2002.121530

DO - 10.1067/mai.2002.121530

M3 - Article

SN - 0091-6749

VL - 109

SP - 271

EP - 273

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

IS - 2

ER -

Array-based diagnostic gene-expression score for atopy and asthma

Abstract

Keywords

UN SDGs

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