Arterial Klotho Expression and FGF23 Effects on Vascular Calcification and Function

Karolina Lindberg; Hannes Olauson; Risul Amin; Arvind Ponnusamy; Regina Goetz; Rebecca F. Taylor; Moosa Mohammadi; Ann Canfield; Karolina Kublickiene; Tobias E. Larsson

doi:10.1371/journal.pone.0060658

Arterial Klotho Expression and FGF23 Effects on Vascular Calcification and Function

Karolina Lindberg, Hannes Olauson, Risul Amin, Arvind Ponnusamy, Regina Goetz, Rebecca F. Taylor, Moosa Mohammadi, Ann Canfield, Karolina Kublickiene, Tobias E. Larsson

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Abstract

Recent studies support a role for FGF23 and its co-receptor Klotho in cardiovascular pathology, yet the underlying mechanisms remain largely elusive. Herein, we analyzed the expression of Klotho in mouse arteries and generated a novel mouse model harboring a vascular smooth muscle cell specific deletion of Klotho (Sm22-KL-/-). Arterial Klotho expression was detected at very low levels with quantitative real-time PCR; Klotho protein levels were undetectable by immunohistochemistry and Western blot. There was no difference in arterial Klotho between Sm22-KL-/- and wild-type mice, as well as no changes in serum markers of mineral metabolism. Intravenous delivery of FGF23 elicited a rise in renal (0.005; p

Original language	English
Article number	e60658
Journal	PLoS ONE
Volume	8
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0060658
Publication status	Published - 5 Apr 2013

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AU - Lindberg, Karolina

AU - Olauson, Hannes

AU - Amin, Risul

AU - Ponnusamy, Arvind

AU - Goetz, Regina

AU - Taylor, Rebecca F.

AU - Mohammadi, Moosa

AU - Canfield, Ann

AU - Kublickiene, Karolina

AU - Larsson, Tobias E.

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Arterial Klotho Expression and FGF23 Effects on Vascular Calcification and Function

Abstract

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