Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation

Shane P. Herbert, Jan Huisken, Tyson N. Kim, Morri E. Feldman, Benjamin T. Houseman, Rong A. Wang, Kevan M. Shokat, Didier Y R Stainier

    Research output: Contribution to journalArticlepeer-review


    Blood vessels form de novo (vasculogenesis) or upon sprouting of capillaries from preexisting vessels (angiogenesis). With high-resolution imaging of zebrafish vascular development, we uncovered a third mode of blood vessel formation whereby the first embryonic artery and vein, two unconnected blood vessels, arise from a common precursor vessel. The first embryonic vein formed by selective sprouting of progenitor cells from the precursor vessel, followed by vessel segregation. These processes were regulated by the ligand EphrinB2 and its receptor EphB4, which are expressed in arterial-fated and venous-fated progenitors, respectively, and interact to orient the direction of progenitor migration. Thus, directional control of progenitor migration drives arterialvenous segregation and generation of separate parallel vessels from a single precursor vessel, a process essential for vascular development.
    Original languageEnglish
    Pages (from-to)294-298
    Number of pages4
    Issue number5950
    Publication statusPublished - 9 Oct 2009


    • Animals
    • Animals, Genetically Modified
    • Aorta/cytology/embryology
    • Arteries/cytology/*embryology
    • Cell Movement
    • Endothelial Cells/cytology/*physiology
    • Ephrin-B2/*metabolism
    • *Morphogenesis
    • Phosphatidylinositol 3-Kinases/metabolism
    • Receptor, EphB4/*metabolism
    • Receptors, Notch/metabolism
    • Signal Transduction
    • Stem Cells/cytology/*physiology
    • Vascular Endothelial Growth Factor A/metabolism
    • Vascular Endothelial Growth Factor Receptor-2/metabolism
    • Vascular Endothelial Growth Factor Receptor-3/metabolism
    • Veins/cytology/*embryology
    • Zebrafish
    • Zebrafish Proteins/metabolism


    Dive into the research topics of 'Arterial-venous segregation by selective cell sprouting: an alternative mode of blood vessel formation'. Together they form a unique fingerprint.

    Cite this