As-resistance in laboratory-reared F1, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil

John Charnock; C. J. Langdon; A. J. Morgan; J. M. Charnock; K. T. Semple; C. N. Lowe

doi:10.1016/j.envpol.2009.05.027

As-resistance in laboratory-reared F1, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil

John Charnock, C. J. Langdon, A. J. Morgan, J. M. Charnock, K. T. Semple, C. N. Lowe

Research output: Contribution to journal › Article › peer-review

Abstract

Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg -1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC 25 sodium arsenate (700 mg kg -1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC 25 concentrations of sodium arsenate (50 mg kg -1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. Crown Copyright © 2009.

Original language	English
Pages (from-to)	3114-3119
Number of pages	5
Journal	Environmental Pollution
Volume	157
Issue number	11
DOIs	https://doi.org/10.1016/j.envpol.2009.05.027
Publication status	Published - Nov 2009

Keywords

Arsenic
Earthworms
Exposure history
Ligand-binding
Resistance

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@article{bfc73f552e994dfeb056e2eebf661820,

title = "As-resistance in laboratory-reared F1, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil",

abstract = "Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg -1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC 25 sodium arsenate (700 mg kg -1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC 25 concentrations of sodium arsenate (50 mg kg -1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. Crown Copyright {\textcopyright} 2009.",

keywords = "Arsenic, Earthworms, Exposure history, Ligand-binding, Resistance",

author = "John Charnock and Langdon, {C. J.} and Morgan, {A. J.} and Charnock, {J. M.} and Semple, {K. T.} and Lowe, {C. N.}",

year = "2009",

month = nov,

doi = "10.1016/j.envpol.2009.05.027",

language = "English",

volume = "157",

pages = "3114--3119",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Elsevier BV",

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T1 - As-resistance in laboratory-reared F1, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil

AU - Charnock, John

AU - Langdon, C. J.

AU - Morgan, A. J.

AU - Charnock, J. M.

AU - Semple, K. T.

AU - Lowe, C. N.

PY - 2009/11

Y1 - 2009/11

N2 - Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg -1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC 25 sodium arsenate (700 mg kg -1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC 25 concentrations of sodium arsenate (50 mg kg -1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. Crown Copyright © 2009.

AB - Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg -1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC 25 sodium arsenate (700 mg kg -1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC 25 concentrations of sodium arsenate (50 mg kg -1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. Crown Copyright © 2009.

KW - Arsenic

KW - Earthworms

KW - Exposure history

KW - Ligand-binding

KW - Resistance

U2 - 10.1016/j.envpol.2009.05.027

DO - 10.1016/j.envpol.2009.05.027

M3 - Article

SN - 0269-7491

VL - 157

SP - 3114

EP - 3119

JO - Environmental Pollution

JF - Environmental Pollution

IS - 11

ER -

As-resistance in laboratory-reared F1, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil

Abstract

Keywords

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