Aspergillus fumigatus catalases: Cloning of an Aspergillus nidulans catalase B homologue and evidence for at least three catalases

Tsuyoshi Takasuka, Nicola M. Sayers, Michael J. Anderson, Emyr W. Benbow, David W. Denning

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The presence of catalases in the water soluble fractions of three Aspergillus fumigatus strains was investigated using nondenaturing and denaturing polyacrylamide gel electrophoresis and Western analysis. Using non-denaturing polyacrylamide gel electrophoresis and staining for catalase activity, three separate catalases were identified. An A. fumigatus catalase gene (catB) was cloned from genomic DNA using the Aspergillus niger catR gene as a probe. Polyclonal antibodies were raised to a glutathione S-transferase-CatB fusion product expressed in Escherichia coli. Western analysis indicated that, under denaturing conditions, the polyclonal antibody recognised a 90-kDa band and under non-denaturing conditions, two separate bands were identified. These results indicate that A. fumigatus in addition to CatB, produces at least two other catalases, one of which is similar in size to CatB. The polyclonal antibody was also used to observe catalase expression in mice, experimentally infected with A. fumigatus. Staining was observed heterogeneously throughout the fungal hyphae. This result indicates that catalase is produced by A. fumigatus during invasive aspergillosis.
    Original languageEnglish
    Pages (from-to)125-133
    Number of pages8
    JournalFEMS Immunology and Medical Microbiology
    Volume23
    Issue number2
    DOIs
    Publication statusPublished - Feb 1999

    Keywords

    • Aspergillus fumigatus
    • Catalase
    • Pathogenic fungi

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