Assembly of transcription factor IIB at a promoter in vivo requires contact with RNA polymerase II

Laura M. Elsby, Amanda J M O'Donnell, Laura M. Green, Andrew D. Sharrocks, Stefan G E Roberts

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The general transcription factor TFIIB has a central role in the assembly of the preinitiation complex at the promoter, providing a platform for the entry of RNA polymerase II/TFIIF. We used an RNA interference (RNAi)-based system in which TFIIB expression is ablated in vivo and replaced with a TFIIB derivative that contains a silent mutation and is refractory to the RNAi. Using this approach, we found that transcriptionally defective TFIIB amino-terminal mutants showed distinct effects on the basis of their ability to compete with wild-type TFIIB in vivo. Moreover, analysis of the TFIIB mutant derivatives by chromatin immunoprecipitation showed that promoter occupancy by TFIIB is dependent on the association with RNA polymerase II. Together, our results support a mode of preinitiation complex assembly in which TFIIB/ RNA polymerase II recruitment to the promoter occurs in vivo.
    Original languageEnglish
    Pages (from-to)898-903
    Number of pages5
    JournalEMBO reports
    Volume7
    Issue number9
    DOIs
    Publication statusPublished - Sep 2006

    Fingerprint

    Dive into the research topics of 'Assembly of transcription factor IIB at a promoter in vivo requires contact with RNA polymerase II'. Together they form a unique fingerprint.

    Cite this