Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review

Kaja Troha; Domen Vozel; Matevž Arko; Apolonija Bedina Zavec; Drago Dolinar; Matej Hočevar; Zala Jan; Matic Kisovec; Boštjan Kocjančič; Ljubiša Pađen; Manca Pajnič; Samo Penič; Anna Romolo; Neža Repar; Vesna Spasovski; Nejc Steiner; Vid Šuštar; Aleš Iglič; Damjana Drobne; Ksenija Kogej; Saba Battelino; Veronika Kralj-Iglič

doi:10.3390/ijms24043420

Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review

Kaja Troha, Domen Vozel, Matevž Arko, Apolonija Bedina Zavec, Drago Dolinar, Matej Hočevar, Zala Jan, Matic Kisovec, Boštjan Kocjančič, Ljubiša Pađen, Manca Pajnič, Samo Penič, Anna Romolo, Neža Repar, Vesna Spasovski, Nejc Steiner, Vid Šuštar, Aleš Iglič, Damjana Drobne, Ksenija KogejSaba Battelino, Veronika Kralj-Iglič^*

^*Corresponding author for this work

Nursing & Midwifery

Research output: Contribution to journal › Review article › peer-review

Abstract

The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.

Original language	English
Article number	3420
Journal	International Journal of Molecular Sciences
Volume	24
Issue number	4
DOIs	https://doi.org/10.3390/ijms24043420
Publication status	Published - 8 Feb 2023

Keywords

centrifugation
extracellular vesicles
fresh-frozen plasma
intercellular signaling peptides and proteins
platelet-rich plasma
platelets
regeneration
small cellular particles
wound healing

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10.3390/ijms24043420

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Troha, K., Vozel, D., Arko, M., Bedina Zavec, A., Dolinar, D., Hočevar, M., Jan, Z., Kisovec, M., Kocjančič, B., Pađen, L., Pajnič, M., Penič, S., Romolo, A., Repar, N., Spasovski, V., Steiner, N., Šuštar, V., Iglič, A., Drobne, D., ... Kralj-Iglič, V. (2023). Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review. International Journal of Molecular Sciences, 24(4), Article 3420. https://doi.org/10.3390/ijms24043420

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title = "Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review",

abstract = "The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.",

keywords = "centrifugation, extracellular vesicles, fresh-frozen plasma, intercellular signaling peptides and proteins, platelet-rich plasma, platelets, regeneration, small cellular particles, wound healing",

author = "Kaja Troha and Domen Vozel and Matev{\v z} Arko and {Bedina Zavec}, Apolonija and Drago Dolinar and Matej Ho{\v c}evar and Zala Jan and Matic Kisovec and Bo{\v s}tjan Kocjan{\v c}i{\v c} and Ljubi{\v s}a Pa{\d}en and Manca Pajni{\v c} and Samo Peni{\v c} and Anna Romolo and Ne{\v z}a Repar and Vesna Spasovski and Nejc Steiner and Vid {\v S}u{\v s}tar and Ale{\v s} Igli{\v c} and Damjana Drobne and Ksenija Kogej and Saba Battelino and Veronika Kralj-Igli{\v c}",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = feb,

day = "8",

doi = "10.3390/ijms24043420",

language = "English",

volume = "24",

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Troha, K, Vozel, D, Arko, M, Bedina Zavec, A, Dolinar, D, Hočevar, M, Jan, Z, Kisovec, M, Kocjančič, B, Pađen, L, Pajnič, M, Penič, S, Romolo, A, Repar, N, Spasovski, V, Steiner, N, Šuštar, V, Iglič, A, Drobne, D, Kogej, K, Battelino, S & Kralj-Iglič, V 2023, 'Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review', International Journal of Molecular Sciences, vol. 24, no. 4, 3420. https://doi.org/10.3390/ijms24043420

TY - JOUR

T1 - Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid

T2 - A Review

AU - Troha, Kaja

AU - Vozel, Domen

AU - Arko, Matevž

AU - Bedina Zavec, Apolonija

AU - Dolinar, Drago

AU - Hočevar, Matej

AU - Jan, Zala

AU - Kisovec, Matic

AU - Kocjančič, Boštjan

AU - Pađen, Ljubiša

AU - Pajnič, Manca

AU - Penič, Samo

AU - Romolo, Anna

AU - Repar, Neža

AU - Spasovski, Vesna

AU - Steiner, Nejc

AU - Šuštar, Vid

AU - Iglič, Aleš

AU - Drobne, Damjana

AU - Kogej, Ksenija

AU - Battelino, Saba

AU - Kralj-Iglič, Veronika

PY - 2023/2/8

Y1 - 2023/2/8

N2 - The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.

AB - The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.

KW - centrifugation

KW - extracellular vesicles

KW - fresh-frozen plasma

KW - intercellular signaling peptides and proteins

KW - platelet-rich plasma

KW - platelets

KW - regeneration

KW - small cellular particles

KW - wound healing

UR - http://www.scopus.com/inward/record.url?scp=85149315326&partnerID=8YFLogxK

U2 - 10.3390/ijms24043420

DO - 10.3390/ijms24043420

M3 - Review article

AN - SCOPUS:85149315326

SN - 1661-6596

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 4

M1 - 3420

ER -

Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review

Abstract

Keywords

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