Automated high-throughput assessment of prostate biopsy tissue using infrared spectroscopic chemical imaging

Paul Bassan, Ashwin Sachdeva, Jonathan H Shanks, Mick D. Brown, Noel W. Clarke, Peter Gardner

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Fourier transform infrared (FT-IR) chemical imaging has been demonstrated as a promising technique to complement histopathological assessment of biomedical tissue samples. Current histopathology practice involves preparing thin tissue sections and staining them using hematoxylin and eosin (H&E) after which a histopathologist manually assess the tissue architecture under a visible microscope. Studies have shown that there is disagreement between operators viewing the same tissue suggesting that a complementary technique for verification could improve the robustness of the evaluation, and improve patient care. FT-IR chemical imaging allows the spatial distribution of chemistry to be rapidly imaged at a high (diffraction-limited) spatial resolution where each pixel represents an area of 5.5 × 5.5 μm2 and contains a full infrared spectrum providing a chemical fingerprint which studies have shown contains the diagnostic potential to discriminate between different cell-types, and even the benign or malignant state of prostatic epithelial cells. We report a label-free (i.e. no chemical de-waxing, or staining) method of imaging large pieces of prostate tissue (typically 1 cm × 2 cm) in tens of minutes (at a rate of 0.704 × 0.704 mm2 every 14.5 s) yielding images containing millions of spectra. Due to refractive index matching between sample and surrounding paraffin, minimal signal processing is required to recover spectra with their natural profile as opposed to harsh baseline correction methods, paving the way for future quantitative analysis of biochemical signatures. The quality of the spectral information is demonstrated by building and testing an automated cell-type classifier based upon spectral features.
Original languageEnglish
Title of host publicationProc. SPIE 9041, Medical Imaging 2014: Digital Pathology, 90410D
EditorsMetin N Gurcan, Anant Madabhushi
Number of pages10
Publication statusPublished - 20 Mar 2014
EventMedical Imaging 2014: Digital Pathology, 90410D - San Diego, California, USA
Duration: 1 Jan 1824 → …


ConferenceMedical Imaging 2014: Digital Pathology, 90410D
CitySan Diego, California, USA
Period1/01/24 → …


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